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Ubiquitin

Ubiquitin is a small regulatory protein that is ubiquitous in eukaryotes. Ubiquitination (or Ubiquitylation) refers to the post-translational modification of a protein by the covalent attachment (via an isopeptide bond) of one or more ubiquitin monomers. Ubiquitin (originally, Ubiquitous Immunopoeitic Polypeptide) was first identified in 1975 as an 8.5 kDa protein of unknown function expressed universally in living cells.


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Re: uH2A Ab source

... lab ran out of the antibody. Any information about alternative sources is highly appreciated. So far, It looks like H2A pulldown and probing for ubiquitin is the only way.

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by Chribu
Thu Jul 10, 2008 4:22 pm
 
Forum: Molecular Biology
Topic: uH2A Ab source
Replies: 6
Views: 1783

immunohistochemistry with mouse skin

Aha! Innervation! You can help me then! Yay! I am planning to apply IHC to investigate innervation of some viscerals. Hypothetically, it involves ubiquitin system, thus I need primary Ab for ubiquitin. Through references, they suggest me to use PGP 9.5. Unfortunately, I am out of that stuff and ...

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by Dr.Stein
Wed Feb 07, 2007 8:04 am
 
Forum: Cell Biology
Topic: immunohistochemistry with mouse skin
Replies: 14
Views: 1261

The Fiber Disease

... You should know you created the other line of genes. Nucelation and cloning. Even skin nerves can be controlled can't they? "New Products for Ubiquitin-Proteasome Research Analyze Ubiquitin pathways with new UpstateĀ® antibodies, proteins, and inhibitors from Millipore. The newest addition ...

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by Skytroll
Sat Nov 04, 2006 4:49 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748656

Antibodies can determine the activation state of an enzyme??

@sdekivit: Are you familiar with "ubiquitin sysem"? I need a help. Thank you in advance ;)

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by Dr.Stein
Thu Oct 26, 2006 8:59 am
 
Forum: Molecular Biology
Topic: Antibodies can determine the activation state of an enzyme??
Replies: 2
Views: 604

tissue-specific protein database?

Hmm I remember I used this for one of my research. I worked on ubiquitin. Give me time to find the site. It is a very good one I believe :?

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by Dr.Stein
Tue Oct 10, 2006 4:06 am
 
Forum: Bioinformatics
Topic: tissue-specific protein database?
Replies: 9
Views: 3911
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