Two-step procedure

... for about a month. After I have amplified the target (flaA) by PCR, I cut the target with DdeI enzyme (Fermentas) for 8 hours according to the procedure suggested by Fermentas. I am using % 4 Nusieve GTG agarose and 100 bp ladder between 100-1000 bp during electrophoresis and rUn the digests ...

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Hi all, I'm planning to use alizarin red staining assay to observe and quantify bone formation on my scaffolds.. Will it be the same procedure as being done on a plate/well. For quantification purpose, we use the de-staining solution and take results using a plate reader. Is it necessary ...

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I have been given a batch of XL1 blue cells. I made them competent using calcium chloride procedure. To test them, i plated them out on agar plate containing: Kanamycin, Tetracyclon, Ampicilin an control. After overnight incubation, there is bacteria colonies in all ...

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... phenotype being selected. Since that phenotype could result from two different genotypes, I wouldn't expect either of them to become fixed ... then you have your answer. (I'm simplifying the necessary experimental procedure a bit, of course.)

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... troubles with the isolation of the bands. I ran the experiment a number of times and didn't always get the same result (despite the exact same procedure and minimal scope for human error). My supervisor suggested that this could be something to do with the splicing nature of the gene.... he ...

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