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Turnover

turnover

The movement of something into, through and out of a place, the rate at which a thing is depleted and replaced.


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Enzymes

Thanks. Have studies on protein turnover been done for specific proteins, or is it just known in general that that is what happens?

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by rk386
Wed Aug 15, 2012 4:04 pm
 
Forum: Molecular Biology
Topic: Enzymes
Replies: 23
Views: 14751

Re: Enzymes

Look for "protein turnover". Here's an introduction. http://en.wikipedia.org/wiki/Protein_turnover

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by jonmoulton
Thu Aug 09, 2012 3:19 pm
 
Forum: Molecular Biology
Topic: Enzymes
Replies: 23
Views: 14751

Enzyme Kinetics - Turnover

... either way in numerous places. However the question i've been given does specifically make a point that it's a homotetramer and asks for the Kcat/turnover number per active site.

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by TJC12
Sun Feb 26, 2012 12:19 pm
 
Forum: Molecular Biology
Topic: Enzyme Kinetics - Turnover
Replies: 2
Views: 3660

Enzyme Kinetics - Turnover

Hi! :) I am having a few problems calculating some required enzyme kinetic values. I've investigated the activity over 3 minutes of alcohol dehydrogenase at varying [S]. I used a spectrophotometer for this. From each of those graphs i've calculated the gradient (A/min) and converted this using the b...

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by TJC12
Sat Feb 25, 2012 9:35 pm
 
Forum: Molecular Biology
Topic: Enzyme Kinetics - Turnover
Replies: 2
Views: 3660

Re: Protein expression but no mRNA

If you have a protein with slow turnover and its expression is inhibited by the activity of the protein (e.g. end-product inhibition) then the protein level could be high but the mRNA very low. If your healthy controls are not growing (e.g. ...

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by jonmoulton
Tue Feb 21, 2012 4:13 pm
 
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 5607
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