Trypsin

Depends on what subset of PBMCs you want to study. In general, blood cells are suspension cells and do not require trypsination, but monocytes are an exception: they adhere to certain types of plastics (which includes most cell culture plates) and must be removed with PBS/EDTA & ...

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... like to monitor the presence of a certain protein on their surface using a labelled antibody. Do I need to treat them as adherent cells using trypsin before adding the mix with the antibody or can I resuspend them pipetting? What do yo think? thanks a lot!

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not all enzymes are proteins, e.g. the ribozyme is RNA trypsin is not cleaved by HCl since it is not in stomach why should be the acid neutralized? Where does the bicarbonate come from?

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... enzyme that has to be cleaved to be activated. Pepsin as well as other enzymes (enzymes are all proteins but not all proteins are enzymes) such as trypsin start off with -ogen at the end of their name. Pepsinogen and trypsinogen. They are cleaved by the hydrochloric acid secreted by parietal cells ...

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... is you mean proteases. These are enzymes (usually proteins themselves) which snip or chew proteins. Enzymes in the stomach are good examples (e.g. trypsin, pepsin). I don't know what you mean by "simple" proteins- small proteins are usually termed peptides (2-15 amino acids or so), although ...

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