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Trypsin

trypsin

(Science: enzyme) serine protease from the pancreas of vertebrates. Cleaves peptide bonds involving the amino groups of lysine or arginine.


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protein extraction from fat breast tissue

... breast tissue. My problem is the following : normal bresat tissue is really fatty and the lipids interfere with my peptides quantification after trypsin digestion. I use the micro BCA from Pierce. The problem is that it seems to overestimate the amount of peptides in normal breast tissue lysates ...

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by Vero36
Sat May 04, 2013 1:18 pm
 
Forum: Cell Biology
Topic: protein extraction from fat breast tissue
Replies: 1
Views: 1748

peripheral mononuclear cells handling

Depends on what subset of PBMCs you want to study. In general, blood cells are suspension cells and do not require trypsination, but monocytes are an exception: they adhere to certain types of plastics (which includes most cell culture plates) and must be removed with PBS/EDTA & ...

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by biohazard
Fri Nov 02, 2012 8:46 am
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2027

peripheral mononuclear cells handling

... like to monitor the presence of a certain protein on their surface using a labelled antibody. Do I need to treat them as adherent cells using trypsin before adding the mix with the antibody or can I resuspend them pipetting? What do yo think? thanks a lot!

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by claire84
Thu Nov 01, 2012 12:18 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2027

drink water, orange juice or Cola before eating?

not all enzymes are proteins, e.g. the ribozyme is RNA trypsin is not cleaved by HCl since it is not in stomach why should be the acid neutralized? Where does the bicarbonate come from?

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by JackBean
Mon Nov 07, 2011 9:29 am
 
Forum: Physiology
Topic: drink water, orange juice or Cola before eating?
Replies: 16
Views: 36264

Re: drink water, orange juice or Cola before eating?

... enzyme that has to be cleaved to be activated. Pepsin as well as other enzymes (enzymes are all proteins but not all proteins are enzymes) such as trypsin start off with -ogen at the end of their name. Pepsinogen and trypsinogen. They are cleaved by the hydrochloric acid secreted by parietal cells ...

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by daniel.kurz
Mon Oct 24, 2011 12:26 am
 
Forum: Physiology
Topic: drink water, orange juice or Cola before eating?
Replies: 16
Views: 36264
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