Trypan blue

... For viability measures, you may want to use erythrosin instead of trypan blue, it is pretty reliable in staining dead cells and leaving live ones unstained but still ...

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... to know if it is possible to get accurate cell viability measures using trypan blue and hemocytometer for counting. thanks

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... to investigate their viability/vitality. I read that double-staining (trypan blue/congo red + Giemsa) is good to differ dead/alive and also the presence of acrosome. Triple-staining ...

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... use PBS 7.4 instead of HBSS to soak my thymocytes, as the staining (trypan blue) is dissolved in PBS. Or is that ok to keep them in HBSS and then pour the dye, which ...

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... staining so I need them alive for some minutes before applying the dye (trypan blue). I think the culture medium is not necessary in this simple experiment. Or?

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