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Transgenic mice

transgenic mice

mice that have a piece of foreign lincor dNA integrated into their genome.


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Conditional knockout mouse

Hello, I am using transgenic mouse where my gene of interest is floxed. When I cross this mouse line with Tamoxifen inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to isolate ...

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by aji01
Wed Feb 20, 2013 11:25 am
 
Forum: Genetics
Topic: Conditional knockout mouse
Replies: 1
Views: 1619

question about LCM

... I am set to perform LCM on PFA-fixed midbrain sections of TH-GPF transgenic mice and encountered some problems. The quantity and quality of RNA isolated from LCM cells ...

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by johnmay
Mon Oct 17, 2011 4:43 pm
 
Forum: Molecular Biology
Topic: question about LCM
Replies: 0
Views: 1184

Problem with transgenic mice

I have sent purified plasmid DNA to mouse transgenic core to develop transgenic mice with that specific DNA piece. Out of about 45 mice that I genotyped, only about 3 mice gave me ...

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by ougadougou
Fri Sep 30, 2011 3:04 am
 
Forum: Genetics
Topic: Problem with transgenic mice
Replies: 2
Views: 7198

Cre-lox transgenic

... mouse to another mouse that contains the Cre 'gene' that is expressed only under a certain, cell-specific promoter. The progeny of these crossed mice should now be conditional knockouts for my gene of interest in only a specific (Cre-targeted) cell subset. My question is: if the lox'd mouse is ...

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by sciencecat
Wed Apr 13, 2011 1:50 am
 
Forum: Molecular Biology
Topic: Cre-lox transgenic
Replies: 2
Views: 1698

Problems with gene knockdown

We use RNAi and Lentiviral transgenesis in our lab to generate transgenic mice. We use luceferase assay to validate the shRNA sequences. The problem is the results don't ...

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by zodiac1505
Tue Sep 08, 2009 11:31 am
 
Forum: Molecular Biology
Topic: Problems with gene knockdown
Replies: 1
Views: 3259
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