Transferases

... Another—remote—possibility is methylation. NdeI doesn’t care about methylation, but XmaI is inhibited by CpG methylation. But the methyl transferases responsible for CpG methylation are eukaryotic enzymes, not bacterial so far as I know. I guess this isn’t a very satisfying solution and ...

See entire post

... Another—remote—possibility is methylation. NdeI doesn’t care about methylation, but XmaI is inhibited by CpG methylation. But the methyl transferases responsible for CpG methylation are eukaryotic enzymes, not bacterial so far as I know. I guess this isn’t a very satisfying solution and ...

See entire post

according to 'Biochemistry' 5th edition by Berg et al it is the cytosine that is methylated on the C-5 by methyl transferases. I am also familiar with methylated adenine to avoid cleavage by restriction endonucleases.

See entire post

... Each enzyme is assigned a recommended name and an enzyme Commission (EC) number. They are divided into six main groups, oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. Any of several complex proteins that are produced by cells and act as catalysts in specific ...

See entire post

... in some detail. The theory states that external magnetic fields can control biological spin-spin proton-proton coupling constants in DNA, RNA, RNA-transferases, and hydrons (H2)). Such spin-spin coupling constants can be stabilized at equilibrium, against the destabilizing external ELFm frequencies. ...

See entire post