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Transferases

transferases

(Science: enzyme) transferases are enzymes transferring a group, for example, the methyl group or a glycosyl group, from one compound (generally regarded as donor) to another compound (generally regarded as acceptor). The classification is based on the scheme donor:acceptor group transferase.

Registry number: EC 2.


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Detoxification

... metabolism of xenobiotics has two phases 1) oxidation, usually done different CYPs 2) conjugation to different substances, this is done by some transferases both steps should lead to increased polarity and thus solubility in water (=blood=urine)

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by JackBean
Wed Nov 04, 2009 5:21 am
 
Forum: General Discussion
Topic: Detoxification
Replies: 5
Views: 3403

Re: problem with cloning/restriction digest

... Another—remote—possibility is methylation. NdeI doesn’t care about methylation, but XmaI is inhibited by CpG methylation. But the methyl transferases responsible for CpG methylation are eukaryotic enzymes, not bacterial so far as I know. I guess this isn’t a very satisfying solution and ...

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by ashlie23
Sat Feb 09, 2008 5:02 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 38785

Re: problem with cloning/restriction digest

... Another—remote—possibility is methylation. NdeI doesn’t care about methylation, but XmaI is inhibited by CpG methylation. But the methyl transferases responsible for CpG methylation are eukaryotic enzymes, not bacterial so far as I know. I guess this isn’t a very satisfying solution and ...

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by blcr11
Fri Feb 08, 2008 3:38 pm
 
Forum: Molecular Biology
Topic: problem with cloning/restriction digest
Replies: 35
Views: 38785

DNA mismatch repair

according to 'Biochemistry' 5th edition by Berg et al it is the cytosine that is methylated on the C-5 by methyl transferases. I am also familiar with methylated adenine to avoid cleavage by restriction endonucleases.

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by sdekivit
Sat Feb 03, 2007 9:02 pm
 
Forum: Molecular Biology
Topic: DNA mismatch repair
Replies: 3
Views: 1758

The Fiber Disease

... Each enzyme is assigned a recommended name and an enzyme Commission (EC) number. They are divided into six main groups, oxidoreductases, transferases, hydrolases, lyases, isomerases and ligases. Any of several complex proteins that are produced by cells and act as catalysts in specific ...

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by al
Sun Dec 24, 2006 4:08 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5479148
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