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Transfer

transfer

The conveyance of something from one place to another.

Origin: L. Ferre = to carry


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What makes dominant allele dominant?

... Or you can have change of a crucial residue to turn off your protein, like in case of Asp to Asn mutations in receptor which impair its ability to transfer phosphates and thus signal the binding. Or you could change the post-translational modification and thus mis-localizing the protein or impairing ...

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by JackBean
Thu Feb 27, 2014 5:20 pm
 
Forum: Genetics
Topic: What makes dominant allele dominant?
Replies: 5
Views: 1084

Re: Gene cloning question

I want to clone a human gene in pET-21a, and transfer to E.coli. I clone the gene in Nde 1 site that it’s sequence is CA/TATG, My question, is The ATG of Nde1 act as a start codon in result I must delete The ATG of my sequence or I can use the ...

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by mohajeri
Thu Feb 06, 2014 10:01 pm
 
Forum: Molecular Biology
Topic: Gene cloning question
Replies: 20
Views: 15557

Re: Western transfer failure

Check the transfer buffer ingredients too, I had this transfer problem once when I forgot to add SDS in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 3991

Re: blank and substrate control give OD reads

... acetate into the substrate 6) incubate @ 50oC, 4hrs in waterbath 7) centrifuge to sediment the substrate 8) pipet 100ul reaction solution and transfer to new 96 deep well plate 9) add 100ul DNS solution, incubate @ 100oC, 5min 10) pipet 100ul and transfer into multiwell plate to read @ OD540 ...

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by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1159

Living or not? (viruses,viroids and prions).

... and its descendants have lost different ones, or each virus picked up different genes from its hosts through a process called horizontal gene transfer. The authors favor the former explanation, because most of the genes specific to one of the two viruses don't look like any gene present in ...

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by Sumanth001
Sun Dec 08, 2013 12:37 pm
 
Forum: Microbiology
Topic: Living or not? (viruses,viroids and prions).
Replies: 15
Views: 4549
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