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Transfection

transfection

(Science: molecular biology) The introduction of dNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA.

Usually accomplished using dNA precipitated with calcium ions though a variety of other methods can be used (e.g. Electroporation). Only about 1% of cultured cells are normally transfected.

Transfection is analogous to bacterial transformation but in eukaryotes transformation is used to describe the changes in cultured cells caused by tumour viruses.


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pfu/cell?

Is it a function of the Virus used in transfection?

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by prithivirajc
Fri Aug 28, 2009 6:01 am
 
Forum: Molecular Biology
Topic: pfu/cell?
Replies: 1
Views: 31

Improving transient CHO protein expression

... are not great compared to the HEKs I was using before. I have played around with ratios and have an optimal ratio (1:1 with 1ug DNA and 1ug MAX transfection reagent). I have also tried putting the cells in hypothermic conditions which appears to have increased the expression by almost double, ...

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by nikinje
Wed Aug 19, 2009 4:41 pm
 
Forum: Cell Biology
Topic: Improving transient CHO protein expression
Replies: 0
Views: 46

Ligation and expression trouble

... plasmid with the same enzymes to make sure there was correct insert. After this visual confirmation, I transfected the plasmid into HEK293 cells. Transfection was performed at 5 different occasions and no positive clones were obtained. SInce the expression of the insert has been frustating and ...

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by solution
Mon Jul 06, 2009 7:36 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Poly A Signals and Fusion Proteins

... A (after GENE) should prevent the extension of the mRNA into the EGFP coding sequence and, thus, prevent the expression of EGFP in cells following transfection. After Cre-mediated recombination, the GENE-polyA will be excised and EGFP will be expressed. Is this correct so far? After transfection ...

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by dgibson
Thu Apr 30, 2009 12:03 am
 
Forum: Molecular Biology
Topic: Poly A Signals and Fusion Proteins
Replies: 0
Views: 191

Re: Mycoplasma and transfection

The Mycoplasma DNA can remain in the cultures for over 6 weeks therefore PCR based detection picks up infection even if its dead. the other tests require live Mycoplasma hence your is it isn't it dilema

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by kazzy
Mon Feb 02, 2009 9:14 pm
 
Forum: Cell Biology
Topic: Mycoplasma and transfection
Replies: 4
Views: 1107
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