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Transfection

transfection

(Science: molecular biology) The introduction of dNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA.

Usually accomplished using dNA precipitated with calcium ions though a variety of other methods can be used (e.g. Electroporation). Only about 1% of cultured cells are normally transfected.

Transfection is analogous to bacterial transformation but in eukaryotes transformation is used to describe the changes in cultured cells caused by tumour viruses.


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Propagation and Transfection of BHK cells?

A and B and negative controls to check for viability of your cells and your media (A) and the process of transfection (B)

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by JackBean
Sun May 04, 2014 12:39 pm
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1146

Propagation and Transfection of BHK cells?

Hi, we are a young biotech company from Germany and have developed a new kind of transfection reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high transfection ...

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by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1146

Overexpression

Dear Aurelie, we are a young biotech company from Germany and have developed a new kind of transfection reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high ...

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by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1444

Overexpression

... technical procedure. So I ask you some questions: - Do you have some advice about the vector providers (I'm in France)? - When I will make the transfection, I will need to select the transfected cells : is it better to use fluorescent proteins (as GFP) or antibiotics ? - A lot of protocol are ...

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by aureliegineste
Wed Sep 04, 2013 7:48 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 1444

Propagation and Transfection of BHK cells?

... going to transfect Baby Hamster Kidney Fibroblasts with an MMP9 expressing plasmid. I wonder to ask what is the expected result of the 3 different transfection conditions and why? :? (i.e. which condition will produce MMP9 expressing cells) 3 conditions: A. Untransfected cell (growth media only) ...

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by biologyonline123
Wed Jul 31, 2013 3:26 pm
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 1146
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