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Transfection

Definition

noun

(molecular biology) The process of deliberate introduction of nucleic acids into a recipient eukaryotic cell


Supplement

Transfection is a process in which molecules such as DNA, RNA, oligonucleotides, and proteins are introduced into the recipient cell, integrating into the chromosomal DNA of the recipient cell. Transfection differs from transduction in a way that DNA transfer can be mediated by non-viral methods in transfection. Examples of non-viral methods are chemical-based transfection (e.g. by precipitating DNA with calcium ions, by highly branched organic compounds such as dendrimers, by cationic liposomes, and cationic polymers such as polyethylenimine), non-chemical methods (e.g. electroporation, cell squeezing, sonoporation, optical transfection, protoplast fusion, hydrodynamic delivery), and particle-based methods (e.g. gene gun, magnetofection, impalecfection, and particle bombardment).

The process is also described as transformation. However, transformation can also refer to the alteration of normal cells to cancer cells (such as caused by tumour viruses).

Transfection is carried out to understand and find a way to treat genetic disorders.

Word origin: Latin trāns (across, on the far side, beyond) + infection

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Results from our forum


Propagation and Transfection of BHK cells?

A and B and negative controls to check for viability of your cells and your media (A) and the process of transfection (B)

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by JackBean
Sun May 04, 2014 12:39 pm
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 2633

Propagation and Transfection of BHK cells?

Hi, we are a young biotech company from Germany and have developed a new kind of transfection reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high transfection ...

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by OliviaZ
Tue Apr 08, 2014 10:48 am
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 2633

Overexpression

Dear Aurelie, we are a young biotech company from Germany and have developed a new kind of transfection reagent called Viromer. Its a synthetic polymer , zero charged with an endosome escape mechanism. The advantages compared to other transfection reagents are: high ...

See entire post
by OliviaZ
Tue Apr 08, 2014 10:45 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 2973

Overexpression

... technical procedure. So I ask you some questions: - Do you have some advice about the vector providers (I'm in France)? - When I will make the transfection, I will need to select the transfected cells : is it better to use fluorescent proteins (as GFP) or antibiotics ? - A lot of protocol are ...

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by aureliegineste
Wed Sep 04, 2013 7:48 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 2973

Propagation and Transfection of BHK cells?

... going to transfect Baby Hamster Kidney Fibroblasts with an MMP9 expressing plasmid. I wonder to ask what is the expected result of the 3 different transfection conditions and why? :? (i.e. which condition will produce MMP9 expressing cells) 3 conditions: A. Untransfected cell (growth media only) ...

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by biologyonline123
Wed Jul 31, 2013 3:26 pm
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 2
Views: 2633
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