Dictionary » T » Transfection

Transfection

transfection

(Science: molecular biology) The introduction of dNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA.

Usually accomplished using dNA precipitated with calcium ions though a variety of other methods can be used (e.g. Electroporation). Only about 1% of cultured cells are normally transfected.

Transfection is analogous to bacterial transformation but in eukaryotes transformation is used to describe the changes in cultured cells caused by tumour viruses.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Overexpression

... technical procedure. So I ask you some questions: - Do you have some advice about the vector providers (I'm in France)? - When I will make the transfection, I will need to select the transfected cells : is it better to use fluorescent proteins (as GFP) or antibiotics ? - A lot of protocol are ...

See entire post
by aureliegineste
Wed Sep 04, 2013 7:48 am
 
Forum: Molecular Biology
Topic: Overexpression
Replies: 1
Views: 668

Propagation and Transfection of BHK cells?

... going to transfect Baby Hamster Kidney Fibroblasts with an MMP9 expressing plasmid. I wonder to ask what is the expected result of the 3 different transfection conditions and why? :? (i.e. which condition will produce MMP9 expressing cells) 3 conditions: A. Untransfected cell (growth media only) ...

See entire post
by biologyonline123
Wed Jul 31, 2013 3:26 pm
 
Forum: Molecular Biology
Topic: Propagation and Transfection of BHK cells?
Replies: 0
Views: 423

cDNA cloning and Lentiviral transfection

I would think that depends on the vector, which you've told us very little about.... The pWPT vector I used for lentiviral transfection has a bacterial promoter, origin of replication, and Ampicillin antibiotic resistance gene, to allow you to amplify it in bacteria. I don't know what ...

See entire post
by BlahBlahBlah
Fri Jul 12, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 548

cDNA cloning and Lentiviral transfection

... I can transfect cells with this vector directly to achieve my goal. But the amount of cDNA I have is very less, so I want to amplify it before transfection. So my question is, Can I transform E.Coli with the vector with cDNA, and obtain copies of it? As in, can I use this vector for cloning ...

See entire post
by rkrishna
Thu Jun 27, 2013 11:22 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 548

ligation error

It sounds like your transfection with pACYCDUET-1 did not work. Perhaps the cells were not competent.

See entire post
by jonmoulton
Tue Feb 19, 2013 5:22 pm
 
Forum: Molecular Biology
Topic: ligation error
Replies: 1
Views: 824
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 20,215 times. 
What links here | Related changes | Permanent link