Home Forum Dictionary Articles Tutorials Books Directory Share your work


Edit page | Page history | Printable version

Dictionary » T » Transfection

Transfection

transfection

(Science: molecular biology) The introduction of dNA into a recipient eukaryote cell and its subsequent integration into the recipient cells chromosomal DNA.

Usually accomplished using dNA precipitated with calcium ions though a variety of other methods can be used (e.g. Electroporation). Only about 1% of cultured cells are normally transfected.

Transfection is analogous to bacterial transformation but in eukaryotes transformation is used to describe the changes in cultured cells caused by tumour viruses.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


ligation error

It sounds like your transfection with pACYCDUET-1 did not work. Perhaps the cells were not competent.

See entire post
by jonmoulton
Tue Feb 19, 2013 5:22 pm
 
Forum: Molecular Biology
Topic: ligation error
Replies: 1
Views: 171

Did not dilute Lipofectamine during Transfection !?

I'm transfecting cells right now and I added 100 uL of OptiMEM to a tube with the reporter plasmid and in the other tube I put 1 uL of Lipofectamine. Proceeded with the protocol and plated them on my P19 cells . . . Totally forgot to dilute the Lipofectamine before I mixed the contents after waiting...

See entire post
by Hicsuntdrac0nis
Thu Sep 06, 2012 11:49 pm
 
Forum: Molecular Biology
Topic: Did not dilute Lipofectamine during Transfection !?
Replies: 0
Views: 977

Cos7 cell transfection issues!

... i was wondering if anyone new any other techniques which are more effective than ecotransfect at transfecting cos7 cells. I know that it was the transfection which didnt work, and i have also repeated the experiment and experienced the same issue. The vector is based on pEGFP. Cheers guys!

See entire post
by cpEGFP4lyf
Thu Mar 29, 2012 1:49 pm
 
Forum: Molecular Biology
Topic: Cos7 cell transfection issues!
Replies: 0
Views: 312

Re: pEGFP-C1

... in this specific promoter and pEGFP-c1 at the same time, then link the specific promoter into pEGFP-C1, replace the cmv. the problem is after transfection NO GFP expressed! WHY this happen? which step is wrong? help me!thanks! Someone in my lab has been having the same problem, she found out ...

See entire post
by daniel.kurz
Mon Nov 21, 2011 2:41 am
 
Forum: Molecular Biology
Topic: pEGFP-C1
Replies: 6
Views: 1635

Re: Biochemical and Molecular Test

... or by transduction (via viral vector). Introducing DNA into eukaryotic cells, such as animal cells, by physical or chemical means is called transfection. Several different transfection techniques are available, such as calcium phosphate transfection, electroporation, microinjection and liposome ...

See entire post
by sykierj
Wed Nov 16, 2011 7:12 am
 
Forum: Molecular Biology
Topic: Biochemical and Molecular Test
Replies: 6
Views: 1574
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 16,564 times. 
What links here | Related changes | Permanent link