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Transcription

Transcription is the first step of gene expression, in which a particular segment of DNA is copied into RNA by the enzyme RNA polymerase. Both RNA and DNA are nucleic acids, which use base pairs of nucleotides as a complementary language that can be converted back and forth from DNA to RNA by the action of the correct enzymes. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. As opposed to DNA replication, transcription results in an RNA complement that includes the nucleotide uracil (U) in all instances where thymine (T) would have occurred in a DNA complement. Also unlike DNA replication where DNA is synthesized, transcription does not involve an RNA primer to initiate RNA synthesis.Although Transcription is nice.


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mRNA vs. cell reporter assay

If the cells autofluoresce, this could confound interpretation of the GTP signal. if you are looking for downregulation of transcription, it takes a while for preexisting GPR to degrade but the mRNA signal is likely to disappear fairly rapidly once transcription stops. For upregulation, ...

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by jonmoulton
Mon Sep 08, 2014 5:27 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

mRNA vs. cell reporter assay

... in a slightly different way: Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase in the expression by Western ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what are the pros and ...

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by aguy
Mon Jul 07, 2014 2:50 am
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 381

What makes dominant allele dominant?

1) each gene can have from one to many alleles 2) it's not only about expression. The difference can be anywhere from transcription to protein post-translational modifications. There can be mutation in the promotor changing significantly expression. If the binding of ribosome will ...

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by JackBean
Thu Feb 27, 2014 5:20 pm
 
Forum: Genetics
Topic: What makes dominant allele dominant?
Replies: 7
Views: 3953

Sigma Factor Functions in Bacterial vs. Eukaryotes

PLEASE HELP! BIOCHEMISTRY HOMEWORK Sigma factor performs four important functions, each required before transcription can begin at a core promoter in bacteria. A) List the four functions. B) Name the transcription factors found in eukaryotes that provide these same four functions. ...

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by sabrina9
Sat Nov 02, 2013 9:49 pm
 
Forum: Molecular Biology
Topic: Sigma Factor Functions in Bacterial vs. Eukaryotes
Replies: 0
Views: 1040
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