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Transcription

Transcription is the first step of gene expression, in which a particular segment of DNA is copied into RNA by the enzyme RNA polymerase. Both RNA and DNA are nucleic acids, which use base pairs of nucleotides as a complementary language that can be converted back and forth from DNA to RNA by the action of the correct enzymes. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, antiparallel RNA strand called a primary transcript. As opposed to DNA replication, transcription results in an RNA complement that includes the nucleotide uracil (U) in all instances where thymine (T) would have occurred in a DNA complement. Also unlike DNA replication where DNA is synthesized, transcription does not involve an RNA primer to initiate RNA synthesis.Although Transcription is nice.


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amplification in no RT control

... to antimicrobials. RNA has been isolated by trizol method and subsequently 'cleaned' on column and finally been DNase treated prior to reverse transcription. I am getting amplification in all of my no RT controls (4 treatments and an untreated), although this is occurring ~20 cycles later than ...

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by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 0
Views: 34

Mutation

... Drosophila DSH gene, and that this mutation alters the protein so that it does not function. This lack of function is the reason for the lack of transcription of the TCF gene. What test or tests would you do to determine if the patient has such a mutation? What results would you expect from your ...

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by hanna92
Thu Oct 16, 2014 4:44 pm
 
Forum: Genetics
Topic: Mutation
Replies: 0
Views: 648

translation from DNA

... to work as information carrier, there would have to be too big changes in the ribosome and everything so it couldn't be possible but rather the transcription system evolved (as that's pretty similar to replication).

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by JackBean
Tue Sep 30, 2014 8:16 am
 
Forum: Molecular Biology
Topic: translation from DNA
Replies: 5
Views: 5253

mRNA vs. cell reporter assay

If the cells autofluoresce, this could confound interpretation of the GTP signal. if you are looking for downregulation of transcription, it takes a while for preexisting GPR to degrade but the mRNA signal is likely to disappear fairly rapidly once transcription stops. For upregulation, ...

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by jonmoulton
Mon Sep 08, 2014 5:27 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 2894

mRNA vs. cell reporter assay

... in a slightly different way: Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase in the expression by Western ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 2894
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