Dictionary » T » Transcriptase




A polymerase that catalyzes the process of reverse transcription.


Reverse transcriptase, or simply transcriptase, makes use of RNA molecule as a template for the synthesis of a complementary DNA strand. It is produced by HIV and other retroviruses in order for them to synthesize DNA from their viral RNA.

It is also found to be involved in the movement of certain mobile genetic elements, such as the Ty plasmid in yeast, in the replication of other viruses such as hepatitis B and possibly in the generation of mammalian pseudogenes.

It was discovered in 1970 by two independent scientists, Howard Temin and David Baltimore.

Synonym: RNA-dependent DNA polymerase, reverse transcriptase.
See also: reverse transcription.

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targeting the Aids virus

... use.This completely destroys HIV-1. But, there are many other mutants out there. We must somehow use an irreversible inhibitor on the reverse transcriptase enzyme.

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by Sumanth001
Fri Sep 27, 2013 12:40 pm
Forum: General Discussion
Topic: targeting the Aids virus
Replies: 3
Views: 3126

overexpression xylanase gene

... - and he is unreachable) that i have to do batch fermentation and *something* PCR. i didnt know either he's talking about real time PCR or reverse transcriptase PCR. what do you think about the clues? how can i relate it to what i have to do to check the activity of over expressed transformant? ...

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by nekonara
Fri Dec 14, 2012 7:50 am
Forum: Molecular Biology
Topic: overexpression xylanase gene
Replies: 2
Views: 2219

PCR from cDNA question

... RNA sample have been heated at 65C for 5min before electrophoresis to eliminate hairpin structures. The cDNA was obtained using iScript reverse-transcriptase, primed by random hexamers. Curiously, the same cDNA gives weird results when taken as template to amplify specific gene products: for ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 4096

delete cDNA stop codon for fusion with fluorescent tag

Hi, Can I use the normal reverse transcriptase kit to obtain the cDNA instead of those cDNA construction kit which contains a specialized vector? Im going to clone it into the vector of my choice. And we decided to change our strategy. We ...

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by arcas
Fri Apr 13, 2012 10:10 am
Forum: Molecular Biology
Topic: delete cDNA stop codon for fusion with fluorescent tag
Replies: 3
Views: 2187

Viral budding

... I have explained the lytic and lysogenic cycles and wanted to mention the reproduction process of retroviruses with the assistance of reverse transcriptase. Taking the HIV virus as an example, upon completion of viral proteins being synthesised inside the host cell, the mature virus can then ...

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by Poggie
Wed Nov 30, 2011 7:36 pm
Forum: Microbiology
Topic: Viral budding
Replies: 1
Views: 2460
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