Titration

A question regarding protoplasts in a dot blot..my protein of interest is in the protoplast which I has isolated and I have been told to do a titration/dilution of it...so I was told do 4x 10 fold diltuin...does this mean if I started with 1:10 my next wld be 1:100, 1:1000, 1:10000/---is this ...

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naa=neutral amino acid aaa=acidic amino acid baa=basic amino acid ......its not stupid

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hi please...could anyone help me??? i have a problem in calculating the net charge for the naa,aaa,baa i dont know where we put +.5,-.5,+1or -1 plzzzzz help me

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... one question...why cant u just do colorimetry or use a spectrophotometer for quantitative analysis?? is it coz its like not accurate or is it coz titration is easier?? n how does titration work here??

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Hello! Its Me again, what a nag am I hehe. I done more research and now I found out that you can do titration to test how much glucose is in the solution, i get this part now. But i need to test starch, carbohydrates and proteins quantitatively as well, so i am not sure, ...

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