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Titrate

titrate

A chemistry lab technique used to find out the concentration of a substance (or concentrations of substances) by slowly putting in a known amount of another substance which can neutralise the effects of the unknown substance. A colour indicator (which reacts with neither) is also included, the colour of the solution changes when all of the unknown substance has been neutralised. The unknown is then inferred by looking at the total amount of known substance it took to neutralise all of it.


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ELISA modification

... continue on with same steps...has anyone done a similar protocol before?...Ideally how much second protein do I need to add, would I just need to titrate out different amounts? Thanks, b_06er

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by biology_06er
Mon Apr 09, 2012 4:37 am
 
Forum: General Discussion
Topic: ELISA modification
Replies: 1
Views: 1316

Dilution-titrate 2 fold definition

Hi there, I have some sera and if it says "dilute sera in PBS-T (titrate 2 fold) starting at 1:200...what exactly does it mean... I'm thinking say I start with a 1:200 1mL sample...take 5uL of sera and add 995uL of PBS-T..that would be my starting diltion ...

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by biology_06er
Tue Nov 22, 2011 9:16 pm
 
Forum: General Discussion
Topic: Dilution-titrate 2 fold definition
Replies: 1
Views: 1567

CFSE proliferation assay

... picks in lymphocyte population. Does anyone encounter this problem before and what could be the reasons. And next question is that I tried to titrate the amount of CFSE using different volume of the same stock and I got sotr of concentration dependent proliferation inhibition: cells cultured ...

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by molbiol
Tue Nov 08, 2011 10:29 am
 
Forum: Cell Biology
Topic: CFSE proliferation assay
Replies: 3
Views: 3550

Preparing buffer solns.

... slow coming back online. As for the 1x TAE, would it be correct just to take a tenth of each of the measurements? Furthermore, when do you know to titrate it? Is that only when you're trying to reach a certain pH of a soln? Thanks in advance!

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by sunnidaze
Sat Sep 03, 2011 6:47 pm
 
Forum: Molecular Biology
Topic: Preparing buffer solns.
Replies: 6
Views: 3645

Re: CHEMISTRY MOLARITY QUESTION

... have been H2SO4 in which case you would have: H2SO4 + 2NaOH ----> Na2SO4 + 2H2O From the equation above you can see that 1 mole of acid would titrate with 2 mole base. We know this because of the 2 in front of the NaOH in other words, if you had spilled one mole of H2SO4 on your skin, you ...

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by MichaelXY
Tue Dec 02, 2008 8:19 am
 
Forum: General Discussion
Topic: CHEMISTRY MOLARITY QUESTION
Replies: 4
Views: 3778
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