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Tissue culture

tissue culture

(Science: cell culture) Originally the maintenance and growth of pieces of explanted tissue (plant or animal) in culture away from the source organism. Now usually refers to the (much more frequently used) technique of cell culture, using cells dispersed from tissues or distant descendants of such cells. An environment created by scientists that is sterile and nutrient rich. The food is to allow the tissue within this culture to easily grow, so that scientists can analyse the tissue more clearly.


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How long will myocytes survive in plasma+saline?

... DMEM or the likes a few years ago in college, as trypsinized adhesion culture and explant chunk from chicken embryo. I've also made hybridomas from mice, so I know about rinsing tissue of red blood cells with PBS, I think I did this with the chicken embryo heart ...

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by nmz787
Tue Oct 15, 2013 9:26 pm
 
Forum: Cell Biology
Topic: How long will myocytes survive in plasma+saline?
Replies: 0
Views: 495

Temperature Variation/Cell Culture Exoeriment

... experiment. What I need: Accurate, adjustable, heating device for cell culture in 35mm tissue culture dishes. Require at least 10 different temperature conditions to fit in a 13inch ...

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by mgarcha
Mon Sep 23, 2013 7:54 pm
 
Forum: Cell Biology
Topic: Temperature Variation/Cell Culture Exoeriment
Replies: 0
Views: 317

Cell culture

I have a question and I need your help badly, I am having a cell culture without a cell line.. only the samples to be tested with DMEM suplemented ... these conditions to my culture knowing that I am culturing in 25-ml tissue culture flasks with filter cap? I need the reply urgently

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by Nadaezz
Sat Sep 21, 2013 1:59 pm
 
Forum: Cell Biology
Topic: Cell culture
Replies: 1
Views: 583

Cell culture - no CO2

I have a question and I need your help badly, I am having a cell culture without a cell line.. only the samples to be tested with DMEM suplemented ... these conditions to my culture knowing that I am culturing in 25-ml tissue culture flasks with filter cap? I need the reply urgently

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by Nadaezz
Sat Sep 21, 2013 12:41 pm
 
Forum: Cell Biology
Topic: Cell culture - no CO2
Replies: 10
Views: 17993

Culturing or viewing cells

... the general procedure assuming you need to get cells from a particular tissue would be to get the tissue, extract the cells, digest ECM..., try culturing them in a flask / Tissue culture plate (depending on the type of cells it can be determined if anchoring is ...

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by jwalin
Fri Aug 30, 2013 2:22 pm
 
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 2275
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