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Time marker

time marker

An instrument that marks the time, usually in seconds or fractions of seconds, on a kymograph record in physiologic experiments.

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Re: How

multiple gene insertion by conventional transformation is very time consuming and required selectable marker genes :( . So I have to do different..................? Do you have any idea ????? :(

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by Helics
Tue Oct 29, 2013 8:52 am
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 2640

Re: Markerless mutants using suicide vector

... deletion of alleles in this bacteria have been reported (a few 10's of times in the literature) although it is reported that illegitimate recombinations ... If we try to, for example, use linear DNA without some selectable marker, we'll just have a difficult time screening for our mutant. One interesting ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 3618

Re: Stomach bloating and weight gain

... I warn everyone to be careful if they try it. I read about the genetic marker of the MTHFR. Some websites report that 3 out of 5 people have it, ... tested but you really do not have too much folate acid. It takes a long time for a Vit B-12 deficiency to show up on lab work. There would be no ...

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by CureIt
Wed Feb 29, 2012 12:30 pm
Forum: Physiology
Topic: Stomach bloating and weight gain
Replies: 453
Views: 2911233

Molecular Cell Biology

... completely stuck :( Problem 2 - Endocytosis of EGF and a fluid phase-marker (HRP) (70 marks) Cells take up extracellular molecules by receptor-mediated ... is at a 500-fold higher concentration than that for EGF) for various times. The cells were then chemically fixed (cross-linked), stained for ...

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by Freckles
Wed Dec 07, 2011 11:24 pm
Forum: Cell Biology
Topic: Molecular Cell Biology
Replies: 5
Views: 5184

CFSE proliferation assay

... have such inhibitory effect on the cells proliferation while being a marker for proliferation? And finally, I decided to test different incubation time of the cells with CFSE. I stained one million newly thawed PBMCs with 2 ul of 5 mM stock ...

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by molbiol
Fri Nov 11, 2011 9:51 am
Forum: Cell Biology
Topic: CFSE proliferation assay
Replies: 3
Views: 4356
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