Home Forum Dictionary Articles Tutorials Books Directory Share your work


Edit page | Page history | Printable version

Dictionary » T » Thrombin

Thrombin

thrombin

(Science: enzyme) protease (34 kD) generated in blood clotting that acts on fibrinogen to produce fibrin. Consists of two chains, A and B, linked by a disulphide bond. B chain has sequence homology with pancreatic serine proteases: cleaves at arg gly.

Thrombin is produced from prothrombin by the action either of the extrinsic system (tissue factor _ phospholipid) or, more importantly, the intrinsic system (contact of blood with a foreign surface or connective tissue). Both extrinsic and intrinsic systems activate plasma factor X to form factor Xa which then, in conjunction with phospholipid (tissue derived or platelet factor 3) and factor V, catalyses the conversion.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Connection between opioid receptors and fatty acid receptors

... this gets in right topic. I used BLAST comparisons semi randomly and found that opioid receptors were at least 20% identical with cannabinoid, thrombin and fatty acid receptors. I don't know if that is new discovery or not. BLAST program can find connections that humans may have not noticed. ...

See entire post
by mhein
Tue Jul 10, 2012 2:00 pm
 
Forum: Evolution
Topic: Connection between opioid receptors and fatty acid receptors
Replies: 4
Views: 3921

thrombin cleavage

A protein of interest is bound to a GST tag. I want to separate the GST tag by using a protease. I found out that thrombin has two cleavage sites in the protein sequence whereas trypsin had more than 20 cleavage sites. I should pick thrombin over trypsin because it is more specific. ...

See entire post
by higherme
Thu Apr 03, 2008 10:53 pm
 
Forum: Molecular Biology
Topic: thrombin cleavage
Replies: 0
Views: 959

Struggling with Vector Cloning Project

... I don't know how far you need to take it, but if you were interested in purifying FAS2, you would have to take the fusion protein and clip it with thrombin and then re-purify the FAS2 (or purify away the GST, whichever is easier).

See entire post
by blcr11
Thu Sep 06, 2007 1:17 am
 
Forum: Molecular Biology
Topic: Struggling with Vector Cloning Project
Replies: 1
Views: 1389

ENZYME CASCADE

... in great detail. A very simple explanation we had in our bio lecture was that cells at the site of injury release clotting factors that convert prothrombin to thrombin, which in turn converts fibrinogen to fibrin, which helps platelets to form a clot.

See entire post
by Jammerz
Tue Apr 24, 2007 4:48 am
 
Forum: Cell Biology
Topic: ENZYME CASCADE
Replies: 5
Views: 6133

pls help- URGENT!!!

... is left behind when the blood clots. ------Summary-------- produce (1) Wound ------------------> thrombokinase ( enzyme ) thrombokinase (2) Prothrombin -------------------> thrombin ( active) (Inactive) Ca2+ ions thrombin (3) Fibrinogen ------------------------> insoluble fibrin threads ...

See entire post
by unscientific
Tue Mar 06, 2007 2:06 pm
 
Forum: Human Biology
Topic: pls help- URGENT!!!
Replies: 4
Views: 3857
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 3,767 times. 
What links here | Related changes | Permanent link