Dictionary » T » Thin layer chromatography

Thin layer chromatography

Definition

noun

A form of chromatography using a thin layer of stationary phase of adsorbent such as silica gel, cellulose, alumina, or any inert material, supported on a glass plate.


Supplement

It is similar to paper chromatography but is faster and provides higher resolution even at smaller samples. Similar to paper chromatography, its uses are primarily for the separation and the identification of the components and to assess the purity of a substance.


Abbreviation: TLC

Variant(s):

Compare:


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: thin layer chromatography

If you've easy access to a UV-Vis spec, you could scrape off the bands, elute them and take some spectra -- then assign the kids into the literature to find likely candidates. It could get complicated if some pigments are co-migrating, but it would also be fun to see if the students come up with tha...

See entire post
by jonmoulton
Mon Nov 28, 2011 10:45 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 2116

Re: thin layer chromatography

Hi Jack, I agree, though the xanthophylls might move a little. pi clouds might give some affinity with the conjugated mobile phase (benzene). - Jon

See entire post
by jonmoulton
Mon Nov 28, 2011 4:59 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 2116

thin layer chromatography

Hi, I just want to make sure I have it correctly, before I'll say the students they are wrong :lol: Anyway, we are doing TLC with extract from red pepper, the stationary phase is silufol (Al2O3) and the mobile phase is benzene. Thus the more hydrophobic pigment, the further it should travel, right? ...

See entire post
by JackBean
Fri Nov 25, 2011 9:36 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 2116

Chromatograms

... unknown. There are several tomes available on chromatography in general which will include paper ... of a discussion about paper chromatography. Thin layer chromatgraphy is still used as a preparative technique ...

See entire post
by blcr11
Thu May 03, 2007 3:19 pm
 
Forum: Botany Discussion
Topic: Chromatograms
Replies: 4
Views: 2618

Chromatography Quick Question

This is paper or thin layer chromatography? And when you say "RNA" and "DNA" are ...

See entire post
by blcr11
Wed Apr 04, 2007 7:14 pm
 
Forum: Molecular Biology
Topic: Chromatography Quick Question
Replies: 3
Views: 1827
View all matching forum results

This page was last modified 15:25, 16 February 2011. This page has been accessed 1,825 times. 
What links here | Related changes | Permanent link