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Thiamine

Thiamine

(Science: chemical) 3-((4-amino-2-methyl-5-pyrimidinyl)methyl)-5-(2- hydroxyethyl)-4-methylthiazolium chloride.

chemical name: Thiazolium, 3-((4-amino-2-methyl-5-pyrimidinyl)methyl)-5-(2-hydroxyethyl)-4-methyl- chloride A B vitamin that prevents beriberi; maintains appetite and growth.More commonly known as vitamin c and found commonly in cereals, thiamine acts as a coenzyme used to breakdown sugars.


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Help: Coating Magnetic Beads with Vitamins

... compounds? Specifically, I am trying to coat Invitrogen Dynabeads (Amine or Carboxylic Acid activated) with vitamin B12 (cobalamin) or B1 (thiamine). I am trying to design an assay that will pull vitamin-binding RNA out of a mixed pool of transcripts. However, I have little chemistry background ...

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by nbogard
Tue Oct 11, 2011 6:20 am
 
Forum: Molecular Biology
Topic: Help: Coating Magnetic Beads with Vitamins
Replies: 1
Views: 2187

Flaws of Life in a Tube

... of DNA -the proteins used in DNA are a bit more complex that the proteins synthesized. Also, DNA is an interviewing of a mass amount of guanine, thiamine, cytosine, and adenine. So much so that when unraveled, it extends form the earth 'till the moon. However, there was nowhere near the amount ...

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by Tomn
Fri Oct 07, 2011 10:17 pm
 
Forum: Evolution
Topic: Flaws of Life in a Tube
Replies: 41
Views: 36725

medium choice confusion

... -, pro-, str r, azi r These mutations confer the following phenotypes on the strains: Genotype Phenotype thi - Thi- cannot synthesise vitamin B1 (thiamine) trp - Trp- cannot synthesise tryptophan his - His- cannot synthesise histidine met - Met- cannot synthesise methionine arg - Arg- cannot synthesise ...

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by ashhh
Sun Aug 21, 2011 2:58 pm
 
Forum: Molecular Biology
Topic: medium choice confusion
Replies: 1
Views: 2923

Screening of a mutation?

... a defined Minimal medium (MM) of course (like M9 glucose) But I am slightly confused as most E. coli will grow on M9 Glucose w/o the need to add thiamine anyway. But if the parental strain do not you might be able to select that way. On the other hand if you are looking for bacteria that suddenly ...

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by canalon
Tue Feb 01, 2011 10:25 pm
 
Forum: Genetics
Topic: Screening of a mutation?
Replies: 2
Views: 1363

Screening of a mutation?

not really, if you grew it on plate with thiamine, all bacteria would grow. On the other hand, if you used plates w/o thiamine, only those, who are able to synthesise it (AKA those with ybc1 mutation) would grow

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by JackBean
Tue Feb 01, 2011 5:39 am
 
Forum: Genetics
Topic: Screening of a mutation?
Replies: 2
Views: 1363
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