Dictionary » T » Terminus

Terminus

terminus

Origin: L. See Term.

1. Literally, a boundary; a border; a limit.

2. The roman divinity who presided over boundaries, whose statue was properly a short pillar terminating in the bust of a man, woman, satyr, or the like, but often merely a post or stone stuck in the ground on a boundary line.

3. Hence, any post or stone marking a boundary; a term. See Term.

4. Either end of a railroad line; also, the station house, or the town or city, at that place.


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N/C terminus

... containing EcoR1/Xho1 sites. When I look at the Pet vector map, am I right in saying my gene is cloned downstream of GFP and attached to the C TERMINUS of GFP. cheers, b_06er N.B...this is the map I am looking at (I can't seem to attach the file hence the web link)..pet32a-c http://www.merck-chemicals.co.uk/life-science-research/vector-table-novagen-pet-vector-table/c_HdSb.s1O77QAAAEhPqsLdcab?PortalCatalogID=merck4biosciences&CountryName=United+Kingdom

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by biology_06er
Sun Dec 04, 2011 5:56 am
 
Forum: General Discussion
Topic: N/C terminus
Replies: 1
Views: 735

Removing MBP tag

... Maybe it would be easier to produce your protein with no tag and just purify through several chromatographic steps. It just may be that the terminus of your protein should be somewhere inside of the structure and thus it cannot be efficiently separated. (however, then the denaturing agents ...

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by JackBean
Fri Oct 21, 2011 12:42 pm
 
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8699

Edman Degradation Problem

... isolated in the lab. You have used two different proteases to cleave the protein. These are the fragments after cleavage (all written with N terminus at the left and C terminus at the right) Protease I ////// Protease II NMLCST ////// STFSTNSC NST ////// STNPGPT NPGPT ////// NPGPTNC NSCST ...

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by pinkerpikachu
Sun Oct 16, 2011 7:35 pm
 
Forum: Molecular Biology
Topic: Edman Degradation Problem
Replies: 1
Views: 3985

RNA Transcription and Sigma Factor

From Wikipedia for "sigma factor": Sigma factors have four main regions that are generally conserved: N-terminus --------------------- C-terminus 1.1 2 3 4 The regions are further subdivided (e.g. 2 includes 2.1, 2.2, etc.) Region 1.1 is found only in "primary sigma ...

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by aptitude
Thu Oct 13, 2011 11:01 pm
 
Forum: Molecular Biology
Topic: RNA Transcription and Sigma Factor
Replies: 1
Views: 2541

Cell Bio

I see that clarifies it. Also, I know it is Tyrosine that attacks carboxypeptidases C-terminus, although how is Tyrosine capable of doing so?

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by spacecowboi
Tue Oct 11, 2011 7:29 pm
 
Forum: Cell Biology
Topic: Cell Bio
Replies: 3
Views: 2781
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