Terminus

... containing EcoR1/Xho1 sites. When I look at the Pet vector map, am I right in saying my gene is cloned downstream of GFP and attached to the C TERMINUS of GFP. cheers, b_06er N.B...this is the map I am looking at (I can't seem to attach the file hence the web link)..pet32a-c http://www.merck-chemicals.co.uk/life-science-research/vector-table-novagen-pet-vector-table/c_HdSb.s1O77QAAAEhPqsLdcab?PortalCatalogID=merck4biosciences&CountryName=United+Kingdom

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... Maybe it would be easier to produce your protein with no tag and just purify through several chromatographic steps. It just may be that the terminus of your protein should be somewhere inside of the structure and thus it cannot be efficiently separated. (however, then the denaturing agents ...

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... isolated in the lab. You have used two different proteases to cleave the protein. These are the fragments after cleavage (all written with N terminus at the left and C terminus at the right) Protease I ////// Protease II NMLCST ////// STFSTNSC NST ////// STNPGPT NPGPT ////// NPGPTNC NSCST ...

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From Wikipedia for "sigma factor": Sigma factors have four main regions that are generally conserved: N-terminus --------------------- C-terminus 1.1 2 3 4 The regions are further subdivided (e.g. 2 includes 2.1, 2.2, etc.) Region 1.1 is found only in "primary sigma ...

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I see that clarifies it. Also, I know it is Tyrosine that attacks carboxypeptidases C-terminus, although how is Tyrosine capable of doing so?

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