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Termination sequence

termination sequence --> termination codon

(Science: molecular biology) The three codons, UAA known as ochre, UAG as amber and UGA as opal, that do not code for an amino acid but act as signals for the termination of protein synthesis.

They are not represented by any tRNA and termination is catalysed by protein release factors. There are two release factors in e. Coli, RF1 recognises UAA and UAG, RF2 recognises UAA and UGA. Eukaryotes have a single gTP requiring factor, eRF.

See: ochre suppressor, amber suppressor.

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... It pauses transcription to allow for capping to occur 4. It facilitates termination of transcription 3? not too sure which else... Ques 2: Which may not be consequences of RNA splicing 1. Insertion of amino acids in the protein sequence 2. Deletion ...

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by 2468
Tue Nov 20, 2012 6:03 pm
Forum: Molecular Biology
Topic: RNA
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... bothering me: If the gene that was inserted into the vector contains a sequence that introns or contains signals which act as terminators to a bacterial host. This results in premature termination, and the recombinant protein may not be processed correctly, be folded ...

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by ericarebelo
Sat Feb 11, 2012 6:32 pm
Forum: Genetics
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Are STOP codons involved in both TRANSCRIPTION & TRANSLATION

... says: "Three of these 64 codons are stop codons that specify the termination of a polypeptide; all the rest code for amino acids" From ... of the DNA strand ends at another special nucleotide sequence called a terminator, which specifies the end of the gene." ...

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by reyrey389
Sun Nov 06, 2011 8:02 am
Forum: Cell Biology
Topic: Are STOP codons involved in both TRANSCRIPTION & TRANSLATION
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Yes. if a transcription terminator sequence is present in an undesirable position, which would lead to premature termination, a particular protein binds to the RNA pol in such a way that it is unable to recognise the ...

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by APmicro
Mon Jun 13, 2011 5:17 am
Forum: Genetics
Topic: Antitermination
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Re: Determining mRNA sequences

Well the article is about nonsense mediated decay and how the pre-termination codon recognition is mediated by a competition between 3' UTR ... antagonize NMD. A mechanism of recruiting these factors could be through sequence-specific binding to a sequence element in the 3' UTR. By using the ...

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by id10t
Thu Mar 03, 2011 8:04 am
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Topic: Determining mRNA sequences
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