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Termination

termination

(Science: molecular biology) stop of mRNA synthesis (i.e., transcription) at the terminator site

top of protein synthesis (i.e., translation) at the stop codon.


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UTRs can play a role in...?

... mRNA are known in some cases to play a role in: A. Regulated mRNA degradation. B. Transcription initiation. C. Assembly of ribosomes. D. Termination of translation. I don't think it's A or C. However, I thought the 5' UTR helped with initiation, and I thought the poly-A tail helped with ...

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by Asynja
Sat Jan 10, 2009 5:07 pm
 
Forum: Genetics
Topic: UTRs can play a role in...?
Replies: 0
Views: 354

Re: dideoxy chain-termination method for sequencing DNA

can anyone help? does anyone know the answer?

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by thewax
Tue Dec 23, 2008 1:22 am
 
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 1654

Re: dideoxy chain-termination method for sequencing DNA

... detector to "sense the color of each fluorescent tag" (pg. 397) - the same procedure of identifying the tags as the dideoxy Cain-Termination Method for sequencing DNA. Wouldn't that also work????? (2) - How to scientists synthesize the ddC, ddG, ddA, or ddT (I mean how do they ...

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by thewax
Wed Dec 17, 2008 5:33 am
 
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 1654

Re: dideoxy chain-termination method for sequencing DNA

thanks MrMistery! :D Just one question: then how does the laser and detector in the current dideoxy chain-termination method for sequencing DNA detect whether the dideoxy nucleotide is actually adenine, thymine, cytosine, or guanine if the laser can't detect the color of the ...

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by thewax
Tue Dec 16, 2008 2:16 am
 
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 1654

dideoxy chain-termination method for sequencing DNA

thewax, That is a legitimate question. You need to understand that in the chain termination method, after doing the amplification in the presence of ddNTPs you separate the molecules by size by electrophoresis. The electrophoresis is sensible enough to separate ...

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by MrMistery
Mon Dec 15, 2008 4:56 am
 
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 1654
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