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Terminase

terminase

(Science: enzyme) involved in assembly of lambda phage; cleaves vegetative concotemeric dNA to generate unit lambda chromosomes; terl encodes large subunit; ters encodes small subunit

Registry number: EC 3.1.-

Synonym: lambda dNA terminase, terl gene product, ters gene product


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The use of Cosmids

Cos sequences are ~200 base pairs long and essential for packaging. They contain a cosN site where DNA is nicked at each strand, 12bp apart, by terminase. This causes linearization of the circular cosmid with two "cohesive" or "sticky ends" of 12bp. (The DNA must be linear ...

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by JackBean
Wed Jan 26, 2011 1:44 pm
 
Forum: Genetics
Topic: The use of Cosmids
Replies: 2
Views: 2516


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