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Terminal transferase

terminal transferase

(Science: enzyme) An enzyme adds a particular nucleotide to the 3' end of DNAstrands.

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Radioactivity Q

... anyone offer any help on the following Q: The enzyme deoxynucleotidyl terminal transferase (TdT) catalyses the addition of dNTPs onto the 3’ ends of double stranded DNA molecules. ...

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by monkey07
Tue May 05, 2009 4:18 pm
Forum: Molecular Biology
Topic: Radioactivity Q
Replies: 0
Views: 2916

how to add a "A" at PCR product ends

... in the manual of TOPO vector, "Another approach was to utilize the terminal transferase activity of Taq DNA polymerase which adds a single 3´-A overhang to each end of ...

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by zhongdianshi
Wed Apr 11, 2007 5:54 pm
Forum: Molecular Biology
Topic: how to add a "A" at PCR product ends
Replies: 6
Views: 22429

The Fiber Disease

... a 30-40 nt-capped RNA derived from the splice leader gene in the 5' terminal ends (Gull 2001). Both the cap structure and the enzymes involved ... trypanosomes as compared to mammalian enzymes. For example, a guanilyl transferase of T. brucei has an extra N-terminal portion (Silva et al. 1998) ...

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by Nadas Moksha
Mon Sep 11, 2006 1:39 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 6246985

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