Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase gene is mapped to the chromosome 10q23-q24 that contains 1,530 cDNA basepairs equivalent to a protein containing 510 amino acids. It is proficient in catalyzing the elongation of DNA strand through toting up of nucleotides from the adjacent solution, such nucleotide accumulation is necessary in some processes especially in lymphocyte development.
Terminal Deoxynucleotidyl Transferase catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligo-deoxyribonucleotides. It requires at least three nucleotides as a primer that shows variable performance depends on the tertiary structure of acceptor RNA end and the nature of nucleotide. It is concentrated in thymus and bone marrow where recombination occurs, thus it is exaggerated by steric exchanges of nucleotide stacking which support the insertion of purines and pyrimidines to make the process less random.
Terminal Deoxynucleotidyl Transferase is useful in immunohistochemistry to display the presence of immature T and B cells that possess the antigen. It is proven that the lack of neonatal TdT is associated to slighter amount of self-reactivity observed in immunoglobins without addition of nucleotides where TdT is not detected in neonatal thymus yet it appears four days after birth.
Gene name: DNTT
Protein name: DNA nucleotidylexotransferase