Template rna

... contamination. Currently I run two negative controls, a -RT and a -template control, both of which are firing. I have changed everything (New; ... with primers designed to span an intron (therefore I no there is cDNA/RNA in my negatives). I have subsequently run a negative with -template and ...

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... of the main theories is that the first main metabolic molecules were RNAs, which can template proteins as well as be active themselves. How you get from such a start to the system ...

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... about PCR. We all know and seems to be common to think of using cDNA as template for PCR. Then my junior had asked me very fundamental question and ... His questions is following - why DNA must be used for PCR and not RNA??? The question arised from following - I did an experiment which isolated ...

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... to PCR a 2kb long segment of a gene out of the cDNA made from total RNA extract but have been experiencing many weird problems so I hope the ... hexamers. Curiously, the same cDNA gives weird results when taken as template to amplify specific gene products: for some proteases, very bright ...

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... a little help to solidify my understanding of Real Time PCR from a mRNA sample (Real Time reverse transcription PCR). The protocol I have in ... later on. Since it's on the 5' end, it will be present on the new template but the polymerase never interacts with the restriction site and ...

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