Template RNA

... From what I understand, now the problem is not to have enough read, it is the assembly. And since they already had an available genome to serve as template, it greatly helps that step too. But it still required probably a few nice computers working hard for those few days. But good for them anyway.

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You'd just use the doubling formula as smellor described(tweak it for your template concentration). Ideally it doubles, of course there's diffusion and other effects

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... resulting in lowered efficiency of transformation in bacteria. Finally, the product DNA is of the same size as the plasmid. Therefore, the template DNA must be eliminated by enzymatic digestion with a restriction enzyme specific for methylated DNA. The template, which for this technique ...

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I need to figure the concentration of amplified DNA after a PCR by using available information like concentration of template DNA, enzyme speed, number of PCR cycles and so forth. Someone told me that i should be able to figure it out myself but im really bad with numbers and math ...

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also, darby, you have it wrong. tRNA ACG would attach to mRNA UGC. UGC comes from DNA ACG TEMPLATE STRAND. The coding strand=nontemplate strand for mRNA UGC is TGC.

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