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Template RNA

Definition

noun

A type of RNA that carries the code or chemical blueprint for a specific protein. In the early stages of protein synthesis, the mRNA is synthesized from a DNA template during transcription.


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RT PCR contamination.

... contamination. Currently I run two negative controls, a -RT and a -template control, both of which are firing. I have changed everything (New; ... with primers designed to span an intron (therefore I no there is cDNA/RNA in my negatives). I have subsequently run a negative with -template and ...

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by georgewiggins
Thu Sep 06, 2012 9:49 pm
 
Forum: Molecular Biology
Topic: RT PCR contamination.
Replies: 2
Views: 3974

Re: question for evolutionary biologist.

... of the main theories is that the first main metabolic molecules were RNAs, which can template proteins as well as be active themselves. How you get from such a start to the system ...

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by scottie
Sat Aug 25, 2012 10:50 pm
 
Forum: Evolution
Topic: question for evolutionary biologist.
Replies: 26
Views: 27305

Question regarding about PCR - DNA or RNA as template

... about PCR. We all know and seems to be common to think of using cDNA as template for PCR. Then my junior had asked me very fundamental question and ... His questions is following - why DNA must be used for PCR and not RNA??? The question arised from following - I did an experiment which isolated ...

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by Xenotoxic
Fri Aug 17, 2012 3:55 am
 
Forum: Molecular Biology
Topic: Question regarding about PCR - DNA or RNA as template
Replies: 1
Views: 3635

PCR from cDNA question

... to PCR a 2kb long segment of a gene out of the cDNA made from total RNA extract but have been experiencing many weird problems so I hope the ... hexamers. Curiously, the same cDNA gives weird results when taken as template to amplify specific gene products: for some proteases, very bright ...

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by vastgenome
Fri Jun 22, 2012 3:55 pm
 
Forum: Molecular Biology
Topic: PCR from cDNA question
Replies: 1
Views: 3081

Real Time reverse transcription PCR Questions

... a little help to solidify my understanding of Real Time PCR from a mRNA sample (Real Time reverse transcription PCR). The protocol I have in ... later on. Since it's on the 5' end, it will be present on the new template but the polymerase never interacts with the restriction site and ...

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by wessH
Sun Jun 03, 2012 2:21 pm
 
Forum: Molecular Biology
Topic: Real Time reverse transcription PCR Questions
Replies: 0
Views: 2386
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