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Template

template

1. A structure that in some direct physical process can cause the patterning of a second structure, usually complementary to it in some sense.

2. (Science: molecular biology) Almost exclusively used to refer to a nucleotide sequence that directs the synthesis of a sequence complementary to it by the rules of Watson crick base pairing. A molecule that provides the structural mould to create similar molecules.


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Taqman probe design using FISH probe (need help)

... at every replication, thus releasing the fluorescence (quenched in intact probe) that will be measured to quantify the starting level of template. Therefore, you can design primers and probes so that they will fall within the sequence recognized by the FISH probe. you can easily find ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 528

Re: Totipotent Stem Cell From Molecular Components

... get the protein? Are you really sure that the stemm cells contain all protein in the same amount? A4: amplicon is the result of PCR. You need some template though, in your case probably the DNA.

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by JackBean
Tue May 13, 2014 5:04 pm
 
Forum: Cell Biology
Topic: Totipotent Stem Cell From Molecular Components
Replies: 2
Views: 6843

Help: Confused with primers in a PCR of a vector?

... we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something I do not really understand. We used the pBS-gene as our template. We carried out four reactions, which are as followed: 1) T7 (forward primer); T3 (reverse primer) 2) T7 (forward primer); coding reverse (reverse ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 2
Views: 4611

Annealing Temparature

... Why do you have such long annealing? What polymerase do you use? What is the GC content of your GOI? How many cycles did you have? What is the template? What concentration did you use?

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by JackBean
Tue Feb 18, 2014 12:40 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 2569

DNA helix and gene

... (read in the opposite direction). However, both strands are required for transcription to mRNA since it's the complement strand that is used as template for it...

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by Cat
Sun Aug 18, 2013 4:36 pm
 
Forum: Molecular Biology
Topic: DNA helix and gene
Replies: 2
Views: 4395
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