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Template

template

1. A structure that in some direct physical process can cause the patterning of a second structure, usually complementary to it in some sense.

2. (Science: molecular biology) Almost exclusively used to refer to a nucleotide sequence that directs the synthesis of a sequence complementary to it by the rules of Watson crick base pairing. A molecule that provides the structural mould to create similar molecules.


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Help: Confused with primers in a PCR of a vector?

... we carried out a PCR reaction of the pBS-gene (our clone). This PCR reaction is something I do not really understand. We used the pBS-gene as our template. We carried out four reactions, which are as followed: 1) T7 (forward primer); T3 (reverse primer) 2) T7 (forward primer); coding reverse (reverse ...

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by keetner
Sat Mar 15, 2014 3:21 am
 
Forum: Molecular Biology
Topic: Help: Confused with primers in a PCR of a vector?
Replies: 0
Views: 65

Annealing Temparature

... Why do you have such long annealing? What polymerase do you use? What is the GC content of your GOI? How many cycles did you have? What is the template? What concentration did you use?

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by JackBean
Tue Feb 18, 2014 12:40 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 350

DNA helix and gene

... (read in the opposite direction). However, both strands are required for transcription to mRNA since it's the complement strand that is used as template for it...

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by Cat
Sun Aug 18, 2013 4:36 pm
 
Forum: Molecular Biology
Topic: DNA helix and gene
Replies: 2
Views: 1981

BL21 colony as a template

... Coli metabolites. I want to amplify a 2kb product from BL21 cells. I do not have genomic DNA of BL21 so, I want to do colony PCR where colony as a template. Can anyone suggest me whether it is possible to amplify the product from colony PCR where colony as a template.

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by sureshG
Mon Jul 15, 2013 10:14 am
 
Forum: Molecular Biology
Topic: BL21 colony as a template
Replies: 0
Views: 383

Promoter PCR primer set problem

... CCGCGG TG AGC AAG TAT ACC AAC CAT -3' (Sacii) Tm 63.3 rv: 5'- gggg TCTAGA CAA TGT ACA CCA ACA TAT AC -3' (Xbai) Tm 56.5 I am using genomic DNA as template and KOD kit. Unfortunately I have been unsuccessful in amplifying the target region. I have tried many annealing temperatures such as 50 up ...

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by bravebeaker
Tue Jul 09, 2013 3:05 am
 
Forum: Molecular Biology
Topic: Promoter PCR primer set problem
Replies: 2
Views: 1897
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