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Temperature-sensitive mutant

temperature-sensitive mutant

A viral mutant that is able to replicate at one portion of a temperature range but not at another, the parent (wild type) strain being able to replicate over the whole temperature range.

Compare: cold-sensitive mutant, quick-stop mutant.


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Cloning, Yeast complementation

... is to verify that you have mutated the proper gene. when you mate the mutant cells you created with cells that have a known mutation in the cdc21 gene, then at the nonpermissive temperature you should still see the mutant phenotype, if you made a mutation in the ...

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by MrMistery
Sat Apr 11, 2009 12:39 pm
 
Forum: Molecular Biology
Topic: Cloning, Yeast complementation
Replies: 1
Views: 2665

DNA replication

no, it's usually because there is a mutant protein that can only fold at the permissive temperature, not at the restrictive temperature.

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by MrMistery
Sun Apr 05, 2009 12:25 pm
 
Forum: Molecular Biology
Topic: DNA replication
Replies: 2
Views: 1335

Re: Genetic Questions - please help!

... mutations in a diploid organism recessive??[/b] :D this is because most mutant alleles are harmful in a constant environment,the existing alleles ... as the mutation allows organism to have a higher tolerance range of temperature.therefore,these organisms are likely to survive,passing down ...

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by chenwithasterisk
Fri Jan 05, 2007 5:24 am
 
Forum: Genetics
Topic: Genetic Questions - please help!
Replies: 4
Views: 4386

The Fiber Disease

... approved for human use, and protein folding agents in reversing the mutant phenotype of familial CJD H187R in a cell model. For an efficient ... three times daily. Interestingly, exposure of PrP187R-GFP cells to low temperature (28°C) or to the chemical chaperones dimethyl sulphoxide (DMSO) ...

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by J Jill
Sun Dec 10, 2006 4:48 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 4988474

The Fiber Disease

... generation. :shock: ___________________________ Genetically engineered mutant of the cyanobacterium Synechococcus PCC 7942 defective in nitrate ... sampling time, thickness of the fiber coating, and the effect of temperature, have been examined. Moreover, the feasibility of headspace SPME ...

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by London
Wed Aug 30, 2006 6:24 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 4988474
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