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Technical

technical

1. Relating to technique.

2. Pertaining to some particular art, science, or trade.

3. In connection with a chemical substance, denoting that the substance contains appreciable quantities of impurities.


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use of a bacterial protease inhibitor cocktail with mammalia

... Blotting, more specifically the use of certain protease inhibitors with the RIPA cell lysis buffer and protease inhibitor cocktail. A Millipore technical representative recommended my using "539134 | Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem" as a good general protease ...

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by rpwdoc
Tue Aug 26, 2014 10:08 pm
 
Forum: Cell Biology
Topic: use of a bacterial protease inhibitor cocktail with mammalia
Replies: 0
Views: 394

qPCR vs Northern Blot

... melting curve looked fine etc. Then I ask you - which result should I believe? Of course I'm going to repeat this, so far I just did technical repetition of qPCR (no change in results), but all hint would be warmly welcomed :). Best regards, Netvhonien

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by Nevthonien
Thu Nov 07, 2013 8:35 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2786

Re: some food for thoughts

... between male and female people? Do the cells just generate differently? -An elementary school student from California Another interesting technical article (if you have access to Nature) is "One tissue, two fates: molecular genetic events that underlie testis versus ovary development" ...

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by ChrisHoStuart
Mon Oct 14, 2013 1:07 am
 
Forum: Evolution
Topic: Gender divison in two opposites is greatest mistake of manki
Replies: 26
Views: 19678

Temperature Variation/Cell Culture Exoeriment

... temperature gauge, and built in thermostat to maintain a set temperature despite environmental fluctuations is a must. Does anyone know of a good technical solution for this set up? I can't think of what to use other than heating plates. Also to clarify I'm heating the cells through the plastic, ...

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by mgarcha
Mon Sep 23, 2013 7:54 pm
 
Forum: Cell Biology
Topic: Temperature Variation/Cell Culture Exoeriment
Replies: 0
Views: 589

Re: possible tfamily trees from four animals

... see a HELP button anywhere to see if it specifies allowed file types, but I'll check around after I submit. I seem to run in to these sorts of technical issues with some frequency. b-n

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by ksharpe212
Tue Sep 10, 2013 7:06 pm
 
Forum: Evolution
Topic: possible tfamily trees from four animals
Replies: 7
Views: 2822
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