Dictionary » T » Target

Target

target

1. An object fixed as goal or point of examination.

2. In the ophthalmometer, the mire.

Synonym: target organ.

4. Anode of an x-ray tube.

See: x-ray.

Origin: It. Targhetta, a small shield


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CRISPR/Cas technology

It works well in mice. About off-targets: it's hyperbole . In mice unless the off target is in LD with your desired mutation, it can be bred out in 1-2 generations and even still, the off targets are primarily in intergenic noncoding sequence ...

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by animodel123
Wed Mar 18, 2015 6:39 am
 
Forum: Genetics
Topic: CRISPR/Cas technology
Replies: 1
Views: 201

Xenobiotic Agents for Biofilm Prevention and Treatment

... to three key factors: (1) the extracellular matrix limits diffusion of molecules such as antibiotics, preventing them from readily accessing the target bacteria; (2) some bacteria within the biofilm are dormant, which diminishes the effectiveness of traditional antibiotics that kill actively ...

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by bioCassie
Tue Mar 03, 2015 5:38 pm
 
Forum: Microbiology
Topic: Xenobiotic Agents for Biofilm Prevention and Treatment
Replies: 0
Views: 252

Questions about DNA dialysis

... I am looking for more information about the theoretical background of dialysis. Can you give me some literature hints, please? Secondly, I know my target DNA concentration, but I know dialysis can lead to dilution and sample loss. How much higher shall I choose the starting concentration? Also, ...

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by protoemics
Thu Feb 05, 2015 7:56 am
 
Forum: Bioinformatics
Topic: Questions about DNA dialysis
Replies: 1
Views: 284

Taqman probe design using FISH probe (need help)

... FISH and Taqman probes have different characteristics. Probes for FISH need to be at least 1 kb long, to hybridize and "highlight" the target chromosomal DNA (metaphase chromosomes or interphase nuclei). Hydrolysis probes (such as Taqman) are very short and used in combination with ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 534

I have got some BIG issues! forgive the long post

... use scientific jargons ( at least it seems to me :p) makes no sense haha. Without further ado: Here they are: Indirect DNA testing : I know yo target the genes that are in severe proximity to our targeted gene and that you make use a fluorescent nucleotide or a tagged reporter molecule that ...

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by saddy51
Sun Jan 04, 2015 10:39 am
 
Forum: Genetics
Topic: I have got some BIG issues! forgive the long post
Replies: 1
Views: 1768
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