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Taq polymerase

Taq polymerase

(Science: enzyme molecular biology) A heat stable dNA polymerase that is normally used in the polymerase chain reaction. It was isolated from Thermobius aquaticus.


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Taqman probe design using FISH probe (need help)

Hi: Although they are both called "probes", FISH and Taqman probes have different characteristics. Probes for FISH need to be at ... (but not so high as a fish probe) and will be hydrolysed by Taq DNA polymerase at every replication, thus releasing the fluorescence (quenched ...

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by protoemics
Wed Feb 04, 2015 8:11 am
 
Forum: Bioinformatics
Topic: Taqman probe design using FISH probe (need help)
Replies: 2
Views: 586

Re: Purpose of a primase/polymerase localized to the cytosol?

Hi, I have some naïve thoughts about this situation: If it is an RNA pol, it may be helping maintain/regulate RNA levels in cytosol by e.g. recharging decaying RNA levels in cytosols for extended translation. If it is a DNA pol, it might be a long surviving vestigial remnant of bacterial ancestry. I...

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by Hrushikesh
Fri Dec 12, 2014 8:40 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1612

Purpose of a primase/polymerase localized to the cytosol?

Hi, I recently read a paper, "PrimPol, an Archaic Primase/Polymerase Operating in Human Cells" by Garcia-Gomez et al. This polymerase was largely localized to the cytosol - I'm wondering if anyone has ideas as to why that would be the case or ...

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by rhardison
Fri Oct 03, 2014 8:51 pm
 
Forum: Molecular Biology
Topic: Purpose of a primase/polymerase localized to the cytosol?
Replies: 2
Views: 1612

Re: Any ideas for vaccine development or anti-viral drugs???

... oligomers (PMOs). A combination of EBOV-specific PMOs targeting sequences of viral mRNAs for the viral proteins (VPs) VP24, VP35, and RNA polymerase L protected rodents in both pre- and post-exposure therapeutic regimens. In a prophylactic proof-of-principal trial, the PMOs also protected ...

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by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12747

Annealing Temparature

... at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, so 1 min can be enough for Phusion, but 4 min will be required for Pfu.

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 2616
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