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Taq polymerase

Taq polymerase

(Science: enzyme molecular biology) A heat stable dNA polymerase that is normally used in the polymerase chain reaction. It was isolated from Thermobius aquaticus.


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Taq Buffer ?

why Taq DNA Polymerase Buffers containing Gelatin ?

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by Helics
Sat Jan 18, 2014 9:36 am
 
Forum: Molecular Biology
Topic: Taq Buffer ?
Replies: 1
Views: 573

How to clone a gene with lots of splicing intermediates?

... fragments that I could then ligate together. I have used proofreading polymerase, Taq, phusion, abgene, and I have then tried (and failed) to isolate, purify and sequence the ...

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by polkadotshorts
Sat Nov 24, 2012 6:49 pm
 
Forum: Genetics
Topic: How to clone a gene with lots of splicing intermediates?
Replies: 6
Views: 3283

Mastermix in PCR

... it does not answer your question. MgCl2 is a required cofactor for the Taq Polymerase to work. The activity of the Taq polymerase is sensitive to the concentration of ...

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by daddyjames
Sun Oct 14, 2012 3:43 pm
 
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 3363

Mastermix in PCR

... of 50 microliter. My mastermix includes 10x butter, dNTPs, MgCl2, and Taq Polymerase, and each of the tube needed to have 13.25 microliter of mastermix solution. But ...

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by klee5533
Tue Oct 02, 2012 1:52 am
 
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 3363

Re: how to add a "A" at PCR product ends

... now a listing on the OpenWetWare Wiki about adding 3' A overhangs using Taq polymerase. The site listing can be found here: http://openwetware.org/wiki/Addition_of_3%27_A_overhangs_to_PCR_products ...

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by CarmeanC
Fri Mar 09, 2012 6:38 pm
 
Forum: Molecular Biology
Topic: how to add a "A" at PCR product ends
Replies: 6
Views: 19621
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