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Tandem

Tandem

term used to describe multiple copies of the same sequence in a polynucleic acid that lie adjacent to one another.


Linkers are short, self-complementary synthetic oligomers which form blunt end duplexes containing a restriction endonuclease recognition sequence. They are used to create cohesive ends on a target dna molecule. Linkers may be obtained with either phosphorylated or unphosphorylated 5' ends for use in different cloning strategies. Blunt-end ligation of phosphorylated linkers to experimental dna fragments produces concatamers of linkers at both ends of each fragment. Digestion with the appropriate restriction endonuclease creates unique cohesive ends.


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Re: how to know a muscle is contracting or relaxing

... when one skeletal muscle relaxes, another opposing muscle is usually contracting. When walking, or running, groups of muscles work in tandem, balancing, striding, pulling the leg back, putting the leg forward, putting the foot, the lower leg the toes, the hips, the ankles, all in the ...

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by Wallyanna
Wed May 09, 2012 10:07 am
 
Forum: General Discussion
Topic: how to know a muscle is contracting or relaxing
Replies: 2
Views: 2649

PCR Accuracy?

I can think of one case where errors might be more likely to occur - when you have a large number of tandem repeats and strand slippage occurs. But I doubt this is going to be a problem for you. In terms of PCR, if you are sequencing afterwards (as you say you are), the accuracy ...

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by cw11
Mon Nov 28, 2011 7:26 pm
 
Forum: Molecular Biology
Topic: PCR Accuracy?
Replies: 6
Views: 2628

Nucleotide sequences

... same, but when restriction enzymes are used to cut them up different fragments are created. Microsatellite repeat sequences: also known as short tandem repeats (STRs) these have a high mutation rate and are used for mapping populations as genetic markers. They're just repeating sequences of a ...

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by Babybel56
Tue May 10, 2011 7:11 pm
 
Forum: Genetics
Topic: Nucleotide sequences
Replies: 4
Views: 2651

STRs, SSRs and microsatellites - Where is the difference?

Simple sequence repeats, short tandem repeats and microsatellites are all the same thing the only difference is the name. They are all repetitive sequences of 1-6 base pairs. It is up to you what you call them but I prefer to use short tandem ...

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by Kezzer
Thu Dec 02, 2010 5:03 pm
 
Forum: Molecular Biology
Topic: STRs, SSRs and microsatellites - Where is the difference?
Replies: 3
Views: 2986

STRs, SSRs and microsatellites - Where is the difference?

... Sequence Repeats) mean a repetition of 1-6 basepairs. For example: AGGAGGAGGAGGAGGAGGAGG But I have also read the same definition for the Short Tandem Repeats (STRs). Now I am wondering if there is any difference? And when do I call it a "microsatellite"? Is a SSR/STR only called "microsatellite" ...

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by Zolt7
Thu Dec 02, 2010 10:43 am
 
Forum: Molecular Biology
Topic: STRs, SSRs and microsatellites - Where is the difference?
Replies: 3
Views: 2986
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