Tag

Typically you would use an antibody to bind to the protein of interest and then a secondary antibody carrying a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent antibody for every antigen you need to detect. ...

See entire post

... possibility that the protein also binds cytoplasmic DNA, but if there is nuclear DNA binding you can nail that down. You could also fluorescent-tag the protein and microinject it or otherwise transfect it into the cytoplasm, then watch where the signal goes. It would be interesting to see if ...

See entire post

the antibody was against tag or your protein?

See entire post

... 06 012 ACG TGC CGT 07 013 ACT TGA AGT 08 020 AGA TCT TCT 09 021 AGC TCG GCT 10 022 AGG TCC CCT 11 023 AGT TCA ACT 12 030 ATA TAT TAT 13 031 ATC TAG GAT 14 032 ATG TAC CAT 15 033 ATT TAA AAT 16 100 CAA GTT TTG 17 101 CAC GTG GTG 18 102 CAG GTC CTG 19 103 CAT GTA ATG 20 110 CCA GGT TGG 21 111 ...

See entire post

Try the other side of the protein or other tag. Sometimes it just doesn't work.

See entire post