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Tag

tag

See: label, tracer.

2. A small outgrowth or polyp.

anal skin tag, a fibrous polyp of the anus.


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Fail to see under physiological stimulation

how was it constitutively active? What's the PGDF? Is your protein endogenous or does it has any tag?

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by JackBean
Thu Aug 22, 2013 11:40 am
 
Forum: Cell Biology
Topic: Fail to see under physiological stimulation
Replies: 6
Views: 1570

I feel like this should be simple!! Help!

... .ffn download? If so, I was able to blast this entire file against the genome, however, the way we want to identify/match genes is with the locus tag identifier and this is not one of the identifiers in this download (the .ffn). Any advice on how best to do this would be greatly appreciated!!!!!! ...

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by smc8339
Wed Jul 17, 2013 6:38 pm
 
Forum: Bioinformatics
Topic: I feel like this should be simple!! Help!
Replies: 2
Views: 1760

Re: Difference between ICC and IF

Typically you would use an antibody to bind to the protein of interest and then a secondary antibody carrying a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent antibody for every antigen you need to detect. ...

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by jonmoulton
Wed Feb 20, 2013 5:20 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 17137

Re: nuclear DNA binding

... possibility that the protein also binds cytoplasmic DNA, but if there is nuclear DNA binding you can nail that down. You could also fluorescent-tag the protein and microinject it or otherwise transfect it into the cytoplasm, then watch where the signal goes. It would be interesting to see if ...

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by jonmoulton
Tue Jan 22, 2013 6:39 pm
 
Forum: Molecular Biology
Topic: nuclear DNA binding
Replies: 1
Views: 1873

low levels of protein but high activity levels

the antibody was against tag or your protein?

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by JackBean
Mon Nov 12, 2012 11:29 pm
 
Forum: Molecular Biology
Topic: low levels of protein but high activity levels
Replies: 3
Views: 1092
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