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T even phage

T even phage

(Science: microbiology) A group of dsDNA bacteriophages of enterobacteria including t2, t4, T6 as opposed to T odd phage (t1, 3, 5 and 7)


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λ Phase Genetic Switch

the λ-phage inserts his DNA by recombination; the integration is site-specific, the recognition-site is found to by 15bp long and is located between the gacactose and tryptophan genes... My thesis: as long as the plasmid DNA ...

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by lenni
Thu Nov 01, 2012 10:08 am
 
Forum: Microbiology
Topic: λ Phase Genetic Switch
Replies: 1
Views: 1397

Question about phage library

Dear all, I was reading about phage library and stumbled on this website (http://phagedisplay.net/). Now, I understand how phage library works, but there is something in the explenation I do not get. Its about this part, and more specific ...

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by poloke
Sat Jun 02, 2012 2:06 pm
 
Forum: Microbiology
Topic: Question about phage library
Replies: 3
Views: 2764

Idea to determine order of assembly of spliceosome

... not know them. If you do not know the proteins, you can hardly have antibody and without antibody it would be difficult to do Western blot ;) The phage promoters should ensure strong expression in bacteria, because that's what phage wants - to express its genes ;)

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by JackBean
Thu May 31, 2012 8:48 am
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7821

Idea to determine order of assembly of spliceosome

... my teacher and he actually suggested I do the precipitation, just as you did. From one thing to another though. What is the purpose of a bacteriophage promoter being ligated in to a vector along with your gene? Is it just to ensure that you get strong expression if you use some kind of phage ...

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by nukeman
Wed May 30, 2012 6:14 pm
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 7821

Re: Just some ideas..

... it using components (genes) that are derived from known viruses, but even that is very difficult and I don't think there have been any major successes with it. You might also find it interesting to look up phage therapy.

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by AstraSequi
Wed Apr 25, 2012 8:49 pm
 
Forum: Microbiology
Topic: Just some ideas..
Replies: 1
Views: 1533
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