T even phage

the λ-phage inserts his DNA by recombination; the integration is site-specific, the recognition-site is found to by 15bp long and is located between the gacactose and tryptophan genes... My thesis: as long as the plasmid DNA ...

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Dear all, I was reading about phage library and stumbled on this website (http://phagedisplay.net/). Now, I understand how phage library works, but there is something in the explenation I do not get. Its about this part, and more specific ...

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... not know them. If you do not know the proteins, you can hardly have antibody and without antibody it would be difficult to do Western blot ;) The phage promoters should ensure strong expression in bacteria, because that's what phage wants - to express its genes ;)

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... my teacher and he actually suggested I do the precipitation, just as you did. From one thing to another though. What is the purpose of a bacteriophage promoter being ligated in to a vector along with your gene? Is it just to ensure that you get strong expression if you use some kind of phage ...

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... it using components (genes) that are derived from known viruses, but even that is very difficult and I don't think there have been any major successes with it. You might also find it interesting to look up phage therapy.

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