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Synthesize

Definition

verb

(chemistry, biochemistry) To produce substance by combining chemical precursors


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Newbie question about the first life

... in a lab. They placed in a glass container what seems to have been the primordial atmosphere, they let sparks fly in it and they managed to synthesize several compounds that you need in order to create life. The conclusion was that lightning had done the same thing in the primitive Earth.

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by piscilactovegetarian
Sun Feb 16, 2014 8:57 pm
 
Forum: Molecular Biology
Topic: Newbie question about the first life
Replies: 14
Views: 8620

Re: mRNA Search for RT-PCR (U to T)

... out of two strands of cDNA then there is a chance that one of the primer will not be complementary to mRNA and it will not bind to mRNA.How can we synthesize cDNA from that mRNA?

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by microniranjan
Sat Oct 05, 2013 9:58 am
 
Forum: Molecular Biology
Topic: mRNA Search for RT-PCR (U to T)
Replies: 8
Views: 5047

Producing unknown protein

... protein sequence to DNA sequence. 2. Codon optimize to the expression system (you have a choice of bacterial or cell free expression systems) 3. Synthesize sequence (IDT can do it for you). 4. Ligate into vector (vector depends on the expression system)/clone vector. 3. Transform and/or express ...

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by Cat
Mon May 20, 2013 1:56 pm
 
Forum: Microbiology
Topic: Producing unknown protein
Replies: 2
Views: 2016

qPCR

1. extracted RNA --> DNase treatment --> RT to synthesize cDNAs 2. use same primers to amplify your target by PCR 3. extract bands from gel and purify target DNA 4. use the purified DNA for making a standard curve. 5. runa a qPCR

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by bravebeaker
Wed Dec 26, 2012 4:47 am
 
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 2040

mRNA transcripts, cis/trans-acting questions

... proteins. You have also isolated two constitutive regulatory mutations that you are calling m1 and m2, respectively. (I.e. both mutants synthesize proteins A and B even without induction.) When you test different genotypes and different partial diploids using F’ strains, you obtain the ...

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by animus31
Fri Nov 09, 2012 11:58 am
 
Forum: Genetics
Topic: mRNA transcripts, cis/trans-acting questions
Replies: 0
Views: 2055
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