Synchronize

we used to starve them of serum to synchronize them. can't you just do that?

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Since Saccharomyces divides by budding, anything that promotes growth promotes budding. But I suspect you want to use synchronized cultures so that most of the viable cells will be budding at roughly the same time. You could go look up E.B. Lewis' papers on cdc genes. I'm sure they ...

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... Would that adaptation speed up if a 'connection' between females exist.By the connection I mean , for example women concentrated in one area will synchronize their menstrual cycles. if they all got with the big one it would happen sooner signed - the big one

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... Would that adaptation speed up if a 'connection' between females exist.By the connection I mean , for example women concentrated in one area will synchronize their menstrual cycles.

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Both these cell lines are great for electroporation; electroporation really doesnt need cells to be in G1/S but you can synchronize them after; you can look this up anywhere. Take care mxj

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