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293 vs 293TN cells (for controls) in WB Analysis

I work with both 293 and 293 TN cells. I know that 293 TN cells differ from that of 293 in that the 293 TN cells express SV40 T Antigen and Neomycin gene products and 293 cells do not. Otherwise, overall protein expression the same. We transfect the cells with a cDNA construct. We ...

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by scsurfer
Tue Jun 29, 2010 11:31 pm
Forum: Cell Biology
Topic: 293 vs 293TN cells (for controls) in WB Analysis
Replies: 0
Views: 2023

Re: Vector with no SV40poly A signal

Here is a vector without a SV40 poly A signal, but you did not mention any other requirements. http://products.invitrogen.com/ivgn/product/15367014 Check invitrogen for other possibilities. http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen?cmd=IVGNcatDisplayCategory&catKey=88101

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by MJD
Thu Oct 01, 2009 12:17 am
Forum: Molecular Biology
Topic: Vector with no SV40poly A signal
Replies: 1
Views: 714

replacing Cells in and outs

... I think that you may be a little confused about what you are after here. We have firefly luciferase vectors under the control of the strong viral SV40 promoter which would be able to express the luciferase gene in plants. However, they will not “glow” unless the substrate for luciferase is present ...

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by mehdi71000
Mon Jun 04, 2007 12:40 pm
Forum: Cell Biology
Topic: replacing Cells in and outs
Replies: 39
Views: 14655

transcriptional cascade

... to switch from, say, early-gene to middle-gene expression, there must be a way to turn off an early gene’s and turn on a middle gene’s expression. SV40 and adenoviruses are probably the most widely studied—I don’t remember too many details of the mechanisms of regulation, but you might find something ...

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by blcr11
Sun Apr 01, 2007 12:33 pm
Forum: Cell Biology
Topic: transcriptional cascade
Replies: 3
Views: 2597

The Fiber Disease

... virus (AcMNPV) followed by convenient restriction sites such as BamHI, Xba I and Asp718. The polyadenylation site of the simian virus 40 ("SV40") is used for efficient polyadenylation. For easy selection of recombinant virus, the plasmid contains the beta-galactosidase gene from E. ...

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by Nadas Moksha
Sat Oct 07, 2006 3:47 am
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5561120
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