Sv40

I work with both 293 and 293 TN cells. I know that 293 TN cells differ from that of 293 in that the 293 TN cells express SV40 T Antigen and Neomycin gene products and 293 cells do not. Otherwise, overall protein expression the same. We transfect the cells with a cDNA construct. We ...

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Here is a vector without a SV40 poly A signal, but you did not mention any other requirements. http://products.invitrogen.com/ivgn/product/15367014 Check invitrogen for other possibilities. http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen?cmd=IVGNcatDisplayCategory&catKey=88101

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... I think that you may be a little confused about what you are after here. We have firefly luciferase vectors under the control of the strong viral SV40 promoter which would be able to express the luciferase gene in plants. However, they will not “glow” unless the substrate for luciferase is present ...

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... to switch from, say, early-gene to middle-gene expression, there must be a way to turn off an early gene’s and turn on a middle gene’s expression. SV40 and adenoviruses are probably the most widely studied—I don’t remember too many details of the mechanisms of regulation, but you might find something ...

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... virus (AcMNPV) followed by convenient restriction sites such as BamHI, Xba I and Asp718. The polyadenylation site of the simian virus 40 ("SV40") is used for efficient polyadenylation. For easy selection of recombinant virus, the plasmid contains the beta-galactosidase gene from E. ...

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