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Sv40


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Biological Immortality and Immortalised Cell lines

... can be used to generate immortalized cell lines. One method is to introduce a viral gene that partially deregulates the cell cycle, such as EBV, SV40 T antigens and HPV-16 E6/7 gene. Artificial expression of key proteins required for immortality is another approach to induce immortalization. ...

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by 2444256703
Thu Jan 22, 2015 8:58 am
 
Forum: Cell Biology
Topic: Biological Immortality and Immortalised Cell lines
Replies: 0
Views: 760

293 vs 293TN cells (for controls) in WB Analysis

I work with both 293 and 293 TN cells. I know that 293 TN cells differ from that of 293 in that the 293 TN cells express SV40 T Antigen and Neomycin gene products and 293 cells do not. Otherwise, overall protein expression the same. We transfect the cells with a cDNA construct. We ...

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by scsurfer
Tue Jun 29, 2010 11:31 pm
 
Forum: Cell Biology
Topic: 293 vs 293TN cells (for controls) in WB Analysis
Replies: 0
Views: 2227

Re: Vector with no SV40poly A signal

Here is a vector without a SV40 poly A signal, but you did not mention any other requirements. http://products.invitrogen.com/ivgn/product/15367014 Check invitrogen for other possibilities. http://products.invitrogen.com/ivgn/en/US/adirect/invitrogen?cmd=IVGNcatDisplayCategory&catKey=88101

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by MJD
Thu Oct 01, 2009 12:17 am
 
Forum: Molecular Biology
Topic: Vector with no SV40poly A signal
Replies: 1
Views: 755

replacing Cells in and outs

... I think that you may be a little confused about what you are after here. We have firefly luciferase vectors under the control of the strong viral SV40 promoter which would be able to express the luciferase gene in plants. However, they will not “glow” unless the substrate for luciferase is present ...

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by mehdi71000
Mon Jun 04, 2007 12:40 pm
 
Forum: Cell Biology
Topic: replacing Cells in and outs
Replies: 39
Views: 16062

transcriptional cascade

... to switch from, say, early-gene to middle-gene expression, there must be a way to turn off an early gene’s and turn on a middle gene’s expression. SV40 and adenoviruses are probably the most widely studied—I don’t remember too many details of the mechanisms of regulation, but you might find something ...

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by blcr11
Sun Apr 01, 2007 12:33 pm
 
Forum: Cell Biology
Topic: transcriptional cascade
Replies: 3
Views: 2781
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