Suspension

I have started using Invitrogen's freestyle CHO cells (without serum, and in suspension) but the protein expression levels are not great compared to the HEKs I was using before. I have played around with ratios and have an optimal ratio (1:1 with 1ug DNA and 1ug ...

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I have been charged with finding out and purchasing a couple urological overhead suspension flasks. From researching online I have been able to find out that a "suspension flask" is a container that allows for cell suspension cultures. When I add "overhead" ...

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You do not need to trypsinize lymphocyte cultures, they grow in suspension and do not attach to the bottom of the well. Some cells, such as monocytes do so, so if you use PBMCs to start your cultures, some cells may attach to the well, but they are not lymphocytes. ...

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If a test tube containing an aqueous suspension of chloroplasts is kept in the dark, what substances would you have to add to the suspension for a 3-carbon sugar phosphate molecule to be formed by photosyntheis? Explain. Any help on the above ...

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... of sky-blue color, and nothing else. A 20% alcohol solution seems to be retaining good color, but I think it may just be keeping the cells in suspension. I'd like to have the majority of the proteins fall out of suspension. Are there any food safe methods of breaking the cell walls that do ...

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