Dictionary » S » Suspension

Suspension

Definition

noun, plural: suspensions

(1) (biochemistry) The state in which the particles of a substance are dispersed but not totally dissolved in a fluid; the substance in this state.

(2) (general) The act or condition of temporary stoppage; an interruption.


Supplement

A suspension contains particles that are mixed by agitation and dispersed in the fluid. When allowed to settle, the particles can be seen at the bottom.

Some drugs are available as oral suspensions. Hence, these drugs need to be taken by first shaking the bottle containing the suspension.


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Calculate the rate of sugar uptake question.

... in umol/g yeast/min for D-Glucose. Ab 520 nm D-Glucose Time: 0 is 0.911 Time: 30 min is 0.345 Sugar uptake. 2.9 mL Kphos buffer pH 7.5 1 mL yeast suspension 0.1 mL 50 mM sugar

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by revision
Wed Mar 20, 2013 6:36 pm
 
Forum: Molecular Biology
Topic: Calculate the rate of sugar uptake question.
Replies: 1
Views: 1160

Problems making chemically competent cells

... at 4°C in a pre-chilled rotor. 6. Resuspend the pellet in RF1 solution. The volume should be 1/3 of the culture volume 7. Incubate the cell suspension on ice for 15 minutes. 8. Pellet the cells as in step 5. 9. Resuspend the pellet in 8ml (1/12.5 of the original culture volume) of RF2. 10. ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 2465

Increasing the amount of bacteria per ml in a suspension

... looked through textbooks and typed every word combination I can possibly think of into google, but nothing. My question is this: You are given a suspension of 10^4 bacteria per ml and need to produce 10^6 per ml (not by growth but by manipulating the suspension). How would you achieve this? Seeing ...

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by Moosh
Wed Nov 14, 2012 12:53 am
 
Forum: Microbiology
Topic: Increasing the amount of bacteria per ml in a suspension
Replies: 2
Views: 1140

peripheral mononuclear cells handling

Depends on what subset of PBMCs you want to study. In general, blood cells are suspension cells and do not require trypsination, but monocytes are an exception: they adhere to certain types of plastics (which includes most cell culture plates) and must be removed ...

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by biohazard
Fri Nov 02, 2012 8:46 am
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 1942

hybridomas

I cannot say for sure without seeing your white dots, but many suspension cells grow in "clumps", meaning that a cell has started to divide there and has generated a lot of daughter cells at that location. Another possibility is that the cells ...

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by biohazard
Mon Feb 06, 2012 7:23 am
 
Forum: Cell Biology
Topic: hybridomas
Replies: 1
Views: 1265
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