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Support medium

support medium

The material in which separation takes place, as in separation of components in electrophoresis.

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Why dialyse DMEM before protein purification (Ni-Affinity)?

Hi, as far as I know expressed intracellulary and then secreted into the medium. The medium is casual DMEM (http://de-de.invitrogen.com/site/de/de/home/support/Product-Technical-Resources/media_formulation.170.html), with high glucose and L-Glutamin ...

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by LittleBird
Sun May 20, 2012 5:55 pm
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3988

Re: Molecular gene (genome) concept scientifically untenable

... encoded by the chemical structure of the hard disk; it only serves as medium for storage. Likewise, some or all of chromosome structure (DNA included) ... about my concept of nonphysical biological information although it is supported by scientific facts, why do you consider molecular gene with ...

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by PAWahid
Thu May 10, 2012 4:54 am
Forum: Molecular Biology
Topic: Molecular gene (genome) concept scientifically untenable
Replies: 12
Views: 38925

Re: Experimental evidence for evolution

... since been propagated by daily 1:100 serial transfer in DM25, a minimal medium containing 25 mg/liter glucose as the limiting resource (2, 22).” ... you have. My question therefore is What is the evidence you call on to support that statement? ( forget the 20,000 bit)

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by jevg
Fri Sep 10, 2010 9:18 pm
Forum: Evolution
Topic: Experimental evidence for evolution
Replies: 103
Views: 194787

What is buoyancy?

... buoyancy is how close you can get to matching the density of your support medium - air, in this case. It relates to how much material and/or power must be put into support.

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by Darby
Mon Aug 09, 2010 5:11 pm
Forum: Evolution
Topic: What is buoyancy?
Replies: 2
Views: 2556


... get N out of the air, even then pure lactose and agar won't likely support your yeast... You can use minimal medium and there are some media without C source. There are chemically defined media used to ...

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by mkwaje
Sat Jan 30, 2010 11:14 am
Forum: General Discussion
Topic: ..
Replies: 7
Views: 2444
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