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Supernatant

supernatant

(Science: chemistry) The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids.


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centrifugation of hemocytes

... ( I tried 5 min at 300g, 10 min at 300g and 5 min at 1000g, in eppendorf and with 96-well plates, flat-bottom and round bottom). I took the supernatant off and tried to resuspend the cells with PBS and then performed my classical viability protocol… But the cells have gone… These velocities ...

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by Thom
Mon Jan 27, 2014 6:41 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 2597

hydroxyproline assay to determine collagen content

Hello ucsfmed2012, How do you treat your samples(100ul of supernatant from each sample)? Do you add 100 μl concentrated HCl (~12N, not provided) in a pressure-tight, teflon capped vial and hydrolyze at 120°C for 3 hours? Thanks! L.

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by Jfangy8
Tue Aug 20, 2013 12:53 am
 
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 8042

Re: Why dialyse DMEM before protein purification (Ni-Affinity)?

I attempted to purge my protein from the unit society supernatant. I transfected my units with my develop to prepare my protein in the supernatant. I gathered the supernatant around the range of 1 litre and after dialysis I subjected my supernatant ...

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by sullivan
Mon Jul 22, 2013 12:28 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2679

protein fractionation

... with regard to a good standard protocol for identifying an unknown protein in the growth media. Again to put it differently, I have protein in the supernatant of the growth media. I just need to identify it. Thanks for all those who read this and Thank you very much for someone who comments. Moti

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by salmonela
Thu May 09, 2013 11:17 am
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 2754

TCA preipitation

... I have looked at a protocol for a cell wall extraction. Here is a simplified version below Wait till OD 0.6 Spin at 5000rpm for 10 mins Remove Supernatant and resuspend pellet in PBS Spin at 5000rpm for ten mins Discard Supernatant and resuspend pellet in 1 mL protoplast buffer. Incubate for ...

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 2396
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