Dictionary » S » Supernatant



(Science: chemistry) The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids.

Please contribute to this project, if you have more information about this term feel free to edit this page

Results from our forum

western blotting of DNA-binding protein of mammalian cell

... in ice for 5 min, followed by passing the lysate through 24 G needle about 5 times...then centrifuged at 12000 g for 15 min..collected the supernatant and pellet.. from the supernatant 40 ul of sample taken and 2X sample buffer added; to the pellet 50ul of 2X sample buffer added, boiled ...

See entire post
by drelamuruganvet
Mon Jan 19, 2015 9:39 am
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 393

centrifugation of hemocytes

... ( I tried 5 min at 300g, 10 min at 300g and 5 min at 1000g, in eppendorf and with 96-well plates, flat-bottom and round bottom). I took the supernatant off and tried to resuspend the cells with PBS and then performed my classical viability protocol… But the cells have gone… These velocities ...

See entire post
by Thom
Mon Jan 27, 2014 6:41 pm
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 5108

hydroxyproline assay to determine collagen content

Hello ucsfmed2012, How do you treat your samples(100ul of supernatant from each sample)? Do you add 100 μl concentrated HCl (~12N, not provided) in a pressure-tight, teflon capped vial and hydrolyze at 120°C for 3 hours? Thanks! L.

See entire post
by Jfangy8
Tue Aug 20, 2013 12:53 am
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 9742

Re: Why dialyse DMEM before protein purification (Ni-Affinity)?

I attempted to purge my protein from the unit society supernatant. I transfected my units with my develop to prepare my protein in the supernatant. I gathered the supernatant around the range of 1 litre and after dialysis I subjected my supernatant ...

See entire post
by sullivan
Mon Jul 22, 2013 12:28 pm
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3987

protein fractionation

... with regard to a good standard protocol for identifying an unknown protein in the growth media. Again to put it differently, I have protein in the supernatant of the growth media. I just need to identify it. Thanks for all those who read this and Thank you very much for someone who comments. Moti

See entire post
by salmonela
Thu May 09, 2013 11:17 am
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 3655
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 26,856 times. 
What links here | Related changes | Permanent link