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Supernatant

supernatant

(Science: chemistry) The soluble liquid fraction of a sample after centrifugation or precipitation of insoluble solids.


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western blotting of DNA-binding protein of mammalian cell

... in ice for 5 min, followed by passing the lysate through 24 G needle about 5 times...then centrifuged at 12000 g for 15 min..collected the supernatant and pellet.. from the supernatant 40 ul of sample taken and 2X sample buffer added; to the pellet 50ul of 2X sample buffer added, boiled ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
 
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
Replies: 0
Views: 393

centrifugation of hemocytes

... ( I tried 5 min at 300g, 10 min at 300g and 5 min at 1000g, in eppendorf and with 96-well plates, flat-bottom and round bottom). I took the supernatant off and tried to resuspend the cells with PBS and then performed my classical viability protocol… But the cells have gone… These velocities ...

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by Thom
Mon Jan 27, 2014 6:41 pm
 
Forum: Cell Biology
Topic: centrifugation of hemocytes
Replies: 5
Views: 5108

hydroxyproline assay to determine collagen content

Hello ucsfmed2012, How do you treat your samples(100ul of supernatant from each sample)? Do you add 100 μl concentrated HCl (~12N, not provided) in a pressure-tight, teflon capped vial and hydrolyze at 120°C for 3 hours? Thanks! L.

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by Jfangy8
Tue Aug 20, 2013 12:53 am
 
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 9742

Re: Why dialyse DMEM before protein purification (Ni-Affinity)?

I attempted to purge my protein from the unit society supernatant. I transfected my units with my develop to prepare my protein in the supernatant. I gathered the supernatant around the range of 1 litre and after dialysis I subjected my supernatant ...

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by sullivan
Mon Jul 22, 2013 12:28 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3987

protein fractionation

... with regard to a good standard protocol for identifying an unknown protein in the growth media. Again to put it differently, I have protein in the supernatant of the growth media. I just need to identify it. Thanks for all those who read this and Thank you very much for someone who comments. Moti

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by salmonela
Thu May 09, 2013 11:17 am
 
Forum: Microbiology
Topic: protein fractionation
Replies: 4
Views: 3655
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