Sucrose

... gene). My vector contains ~1.2kb of homologous sequences that flank both sides of my gene (~2.4kb homologous regions total). After selection on sucrose, I've found that 100% of my clones so far are WT and not the deleted mutant as desired. I'm wondering if anyone would have any suggestions on ...

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... is gram neg, med rods. However, the tests I've done are coming back negative with two being somewhat quesitonable. Lactose +/+ Glucose +/+ sucrose +/+ Citrate - green - (so this is -) Gelatin showed no liquifaction, so this is - EMB agar - no reaction, so - Catalase - ( no bubbles) PAD ...

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... hemolytic EMB: Colorless growth (does not ferment lactose) MS Agar: No growth TSI: Yellow butt, yellow slant (ferments glucose, lactose and/or sucrose) (I am also wondering does this mean it does ferment lactose? Or can it mean that it ferments sucrose instead of lactose?) Nitrate Reduction: ...

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As a general answer - yes plants contain different levels of sucrose. Hence, why we grow sugarcane and sugar beets to produce sugar. I am confused as to why different levels of sucrose would affect the survival of the leaf if the leaf is cut? The sucrose ...

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... or will it not be enough). To do this, I need different plants to test it on. My idea was to judge which plants to use if they had a difference in sucrose (or any kind of sugar for that matter) levels. If anyone has a better idea for how I should judge which plants to use, please let me know. I ...

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