Subtilisin

... a specific kind of peptide bonds it breaks. Examples of proteases include: fungal protease, pepsin, trypsin, chymotrypsin, papain, bromelain, and subtilisin." http://www.enzymeessentials.com/HTML/proteases.html Take your pick, mix and match: http://www.promega.com/techserv/enzymes/default.htm ...

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... of the reagents are as such: Cell resuspension solution: Tris-HCl EDTA RNase A Cell lysis solution: NaOH SDS Alkaline protease Protease enzyme subtilisin (produced by Bacillus licheniformis ) Neutralising solution: Potassium acetate Tris- HCl EDTA Column wash solution: potassium acetate Tris- ...

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