Substrate specificity

... restriction enzyme and I know that restriction enzyme recognize its substrate by recognizing distortion of the recognition site. And then the ... the subsequent binding of the magnesium ion account for the catalytic specificity of more than 1,000,000-fold that is observed for EcoRV endonuclease. ...

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... here you can see, that there are several kinds of them differing in substrate specificity. Alpha breaks the chain in middle releasing oligosaccharides (having several Glc ...

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... help with. how you would go about changing the catalytic activity or substrate specificity of an enzyme whose gene you have isolated. Why would you want to do this?

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... of function, then I have 2 examples for you: Solvent can inlfuence the substrate specificity of some enzymes (Restriction enzymes sometimes show star activity when used with ...

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... sure I have the best answer or not. In my mind the two can be related. Specificity would be a discriminatory function--an enzyme can discriminate between two stereoisomers of a substrate and so is specific, preferring to use one isomer over the other. Affinity ...

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