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Substrate inhibition

substrate inhibition

inhibition of an enzyme activity by a substrate of the reaction catalyzed by that enzyme; often, this type of inhibition occurs at elevated substrate levels in which the substrate is binding to a second, non-active site on the enzyme.


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Amyloid beta (A4) precursor protein degradation products

... of relative equilibrium and there is competition in the role of the substrate.Physiological conditions, the main enzymatic pathways of α secretion, ... Alzheimer's disease in the pathological process, including through the inhibition of proteasome activity and the promotion of tau protein aggregation ...

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by Anonymous
Tue Jun 12, 2012 3:11 am
 
Forum: General Discussion
Topic: Amyloid beta (A4) precursor protein degradation products
Replies: 1
Views: 6788

enzyme inhibition assay

... I would like to calculate Ki of an inhibitor using a FRET inhibition assay.The FRET assay are run for 30 minutes at 25 °C. At the end ... of reagents and incubating the enzyme with inhibitor before adding substrate, this humps do not appear. Any suggestions? Thank you in advance!!

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by claire84
Thu Jan 12, 2012 11:15 am
 
Forum: Molecular Biology
Topic: enzyme inhibition assay
Replies: 0
Views: 994

enzymatic inhibition assay

Hello, I am new to your forum. I have to test the inhibition potency of same potential inhibitors against a metalloprotease, known ... at 25°C with a LF concentration of 10 nM. After the addition of LF to the substrate, the release of p-nitroaniline was monitored recording the absortion ...

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by claire84
Mon Aug 23, 2010 2:00 pm
 
Forum: Molecular Biology
Topic: enzymatic inhibition assay
Replies: 2
Views: 2730

Noncompetitive inhibition and allosteric inhibition

... a big test coming up soon. What's the difference between noncompetitive inhibition and allosteric inhibition????????? The progress I made on this ... inhibition, the enzyme contains more than one active site and the substrates do not resemble each other. However, when one substrate binds ...

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by thewax
Wed Dec 24, 2008 11:14 pm
 
Forum: Molecular Biology
Topic: Noncompetitive inhibition and allosteric inhibition
Replies: 2
Views: 31085

ETC inhibitors;acetate in glycogen;ischemia;melonate;HELP

... B and cyanide both inhibit oxidative phosphorylation when the substrate is either pyruvate or succinate. Dinitrophenol can distinguish ... that the citric acid cycle could be inhibited by malonate and that this inhibition could be overcome by raising the oxaloacetate concentration. Explain ...

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by psychic_pizza
Sun Mar 23, 2008 7:30 am
 
Forum: Molecular Biology
Topic: ETC inhibitors;acetate in glycogen;ischemia;melonate;HELP
Replies: 1
Views: 3771
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