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Substrate-level phosphorylation

substrate-level phosphorylation

synthesis of high-energy phosphate bonds through reaction of inorganic phosphate with an activated (usually) organic substrate.


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Re: ATP

Substrate phosphorylation is when phosphate group is transfered from some phosphorylated compound to ADP (like in glycolysis). ...

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by Borisfen
Thu May 01, 2008 9:21 am
 
Forum: Cell Biology
Topic: ATP
Replies: 7
Views: 3955

ETC inhibitors;acetate in glycogen;ischemia;melonate;HELP

... would be appreciated 1)Oligomycin B and cyanide both inhibit oxidative phosphorylation when the substrate is either pyruvate or succinate. Dinitrophenol can distinguish between these inhibitors. ...

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by psychic_pizza
Sun Mar 23, 2008 7:30 am
 
Forum: Molecular Biology
Topic: ETC inhibitors;acetate in glycogen;ischemia;melonate;HELP
Replies: 1
Views: 879

The Fiber Disease

... hydrolase families. Proteins are designated according to their substrate specificity "The thermophilic filamentous bacterium Thermobifida ... of the complexed type, cell-substrate attachment, coupling of sugar phosphorylation to hydrolysis of ß-glucosidic bonds (to at least some extent), ...

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by Nadas Moksha
Tue Dec 19, 2006 7:35 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748642

Phosphorylation of Cdks

I have a novel protein which is thought to be a substrate of the Cdk1 kinase. Can anyone tell me whats the benefit in knowing the specific site of phosphorylation?? Thanks, in advance! :)

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by medbio
Sun Dec 10, 2006 1:50 am
 
Forum: Cell Biology
Topic: Phosphorylation of Cdks
Replies: 1
Views: 476

non-radioactive ATP analog for kinase assay?

Hello I am trying to check wether a certain protein is a substrate for the mTOR kinase in mammalian tissue culture (NIH 3T3 cells). I ... ATP and then IP the protein and see if mTOR inhibition would change its phosphorylation status. I really don't want to use such a high amount of 32P-ATP, ...

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by unibas
Tue Aug 09, 2005 8:06 am
 
Forum: Cell Biology
Topic: non-radioactive ATP analog for kinase assay?
Replies: 1
Views: 825
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