Substrate-level phosphorylation

Substrate phosphorylation is when phosphate group is transfered from some phosphorylated compound to ADP (like in glycolysis). ...

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... would be appreciated 1)Oligomycin B and cyanide both inhibit oxidative phosphorylation when the substrate is either pyruvate or succinate. Dinitrophenol can distinguish between these inhibitors. ...

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... hydrolase families. Proteins are designated according to their substrate specificity "The thermophilic filamentous bacterium Thermobifida ... of the complexed type, cell-substrate attachment, coupling of sugar phosphorylation to hydrolysis of ß-glucosidic bonds (to at least some extent), ...

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I have a novel protein which is thought to be a substrate of the Cdk1 kinase. Can anyone tell me whats the benefit in knowing the specific site of phosphorylation?? Thanks, in advance! :)

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Hello I am trying to check wether a certain protein is a substrate for the mTOR kinase in mammalian tissue culture (NIH 3T3 cells). I ... ATP and then IP the protein and see if mTOR inhibition would change its phosphorylation status. I really don't want to use such a high amount of 32P-ATP, ...

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