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Substrate

Definition

noun, plural: substrates

(geology)

A substratum or an underlying stratum.

(chemistry)

The substance acted upon by an enzyme.

(biology)

The earthy material in which an organism lives, or the surface or medium on which an organism grows or is attached.

(marine biology)

The material (e.g. dirt, rocks, sand, gravel) in the bottom of a marine habitat, or one that forms the bed of a stream (or of an aquarium); the source of food for some microorganisms.


Supplement

Word origin: L. Stratum = layer.

Related terms: substrate inhibition, substrate-level phosphorylation, substrate specificity, suicide substrate, enzyme-substrate complex.

Synonym: substratum.


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Results from our forum


Re:

... which is I used for prevent any contamination in the reaction but I think there is no difference after added this reagent, means that OD blank and substrate control still higher.

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by intandiana
Sun Jan 19, 2014 5:59 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1217

Re: blank and substrate control give OD reads

You centrifuge the reactions in the 96-well plate and then in that you measure absorbance? How can you do that if the bottom is blocked by your substrate? Can you describe your reactions and controls in more detail including some example absorbances so we can make better idea what you have in ...

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by intandiana
Sun Jan 19, 2014 5:57 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1217

Re: blank and substrate control give OD reads

Here's another approach. Set up a pair of tubes identically, containing everything but the xylanase. Check their OD. Add xylanase to one of the tubes and mix. Check the OD again. That way you control for other potential errors, such as a difference in buffer concentration or a variation in tube thi...

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by intandiana
Sun Jan 19, 2014 5:45 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1217

Re: blank and substrate control give OD reads

Is there anything in the buffer that absorbs light at the wavelength you are using for your OD readings? It might be worth running the buffer in a spectrophotometer and comparing against water at wavelengths near your OD measurement. Dear jonmoulton, thanks for your reply. >>>my buffer is ...

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by intandiana
Sat Jan 18, 2014 8:39 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1217

Re: blank and substrate control give OD reads

You centrifuge the reactions in the 96-well plate and then in that you measure absorbance? How can you do that if the bottom is blocked by your substrate? Can you describe your reactions and controls in more detail including some example absorbances so we can make better idea what you have in ...

See entire post
by JackBean
Fri Jan 17, 2014 10:05 am
 
Forum: Molecular Biology
Topic: blank and substrate control give OD reads
Replies: 9
Views: 1217
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