Dictionary » S » Substitute



1. Anything that takes the place of another.

2. In psychology, a surrogate.

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Methylene Blue Solution use in staining variety of bacteria?

... turn green), 2 changes of 100% alcohol (10 dips each) (alcohol used for dehydration removes some of the stain). 5. Clear in xylene or xylene substitute. 6. Mount with resinous mounting medium.

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by scytek
Sun Feb 22, 2015 9:22 am
Forum: General Discussion
Topic: Methylene Blue Solution use in staining variety of bacteria?
Replies: 0
Views: 311

Gene deletion

Use the same protocol, just modify it - substitute Luciferase or other fluorescent protein gene in place of an antibiotic resistance gene.

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by Cat
Sun Sep 15, 2013 3:47 pm
Forum: Molecular Biology
Topic: Gene deletion
Replies: 5
Views: 3518

Problems with FluoroJade staining

... the samples to put them into a minute of dH20 water in step three… Can I skip step 1 and 2? B) Can I use clearing agent before drying? Or can I substitute drying by dehydrating by ethanol baths? I also wonder if I can substitute xylene by histoclear? C) Why are my sections getting bubbles and ...

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by fabulousparrot
Mon May 20, 2013 1:47 pm
Forum: Molecular Biology
Topic: Problems with FluoroJade staining
Replies: 0
Views: 2134

Cuticle, spores, vascular, plant evolution

... vascular tissues. The early plants (specifically the bryophytes) were considered to lack vascular tissues, yet they do have representative substitute tissues that served the same purpose like leptoids and hydroids in mosses (these were not considered vascular tissues because they lack some ...

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by Chartreuse
Sat Feb 18, 2012 1:06 am
Forum: Botany Discussion
Topic: Cuticle, spores, vascular, plant evolution
Replies: 1
Views: 3320

Reasons for Skepticism on the Endosymbiosis?

... genes without affecting existing functions? The existence of obligate bacterial endosymbionts in some present eukaryotes is often presented as a substitute for a mechanism, but they remain bacteria and give not rise to new organelles. So, before we can speak of the endosymbiotic as a testable ...

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by SuperfluousAvatar
Thu Jan 12, 2012 7:43 pm
Forum: Cell Biology
Topic: Reasons for Skepticism on the Endosymbiosis?
Replies: 0
Views: 7665
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