Streptavidin

... Pharmingen - Cat # 550397 CD3 by BD Bioscience Pharmingen - Cat # 550367 I used the DAKO Labs' developer kit (with the Biotinylated Link and Streptavidin-HRP) and DAB chromogen for the staining, and Mayer's Hemotoxylin for the counter-stain. The results were not very good. The Pharmingen ...

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... then subjected to tyramide signal amplification according to the manufacturer's instructions (TSA indirect, NEN Life Sciences, Boston, MA). Cy5-streptavidin (Amersham, Piscataway, NJ) at 1 mg/mL was diluted 1:500 and added to the slides for fluorescence signal detection. Tissue sections were ...

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... is demonstrated by detecting DNA targets . Single-strand DNA (ssDNA) probes labeled with biotin were immobilized onto microbeads coated with streptavidin . The DNA-functionalized microbeads were packed into each of three microchambers by injection through inlet wells . Three oligonucleotides ...

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... (7249 bp) DNA) 2.What are the denaturing agents that can be used to denature a double strand (e.g. Lambda DNA) to get a single strand, preserving streptavidin-biotin bonds? Also what’s the ideal pH and salt concentration to keep the single strand intact after the double stranded DNA was denatured? ...

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... Some modifications to the original method include hybridization of biotinylated gene-specific degenerate oligo and subsequent stringent washes of streptavidin coated paramagnetic beads after affinity separation. A PCR with nested primer from second RnaseH motif followed this capture. A study of ...

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