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Streptavidin

streptavidin

(Science: protein) analogue of avidin. A protein isolated from streptomycetes avidinii that has a high affinity for biotin. Used to detect biotin markers.


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electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1222

Re: Determining an Ig percentage from crude serum w/ELISA

... The OVA-IgE uses a monoclonal antibody to mouse IgE heavy chain attached to the wells as a capture antibody and then biotinylated ovalbumin and streptavidin-peroxidase in the presence of substrate is used as a detection antibody. If you go to each on our website you can view the product insert ...

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by researcher4life
Fri May 07, 2010 6:42 pm
 
Forum: Molecular Biology
Topic: Determining an Ig percentage from crude serum w/ELISA
Replies: 2
Views: 2112

Spin-down speed for HEK293 after aantibody binding?

Hello, I do antibody binding test with anti-human-IgG-PE and for the other assay with biotinylated antibody and streptavidin-apc-cy7 as the second AB. I am using 293T cells for the assay. Since the pellett is very loose I would like to increase the centrifugation speed during the ...

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by MattNYC
Mon Oct 19, 2009 7:17 pm
 
Forum: Molecular Biology
Topic: Spin-down speed for HEK293 after aantibody binding?
Replies: 2
Views: 3199

Immunohistochemistry HELP--paraffin vs frozen sections

... Pharmingen - Cat # 550397 CD3 by BD Bioscience Pharmingen - Cat # 550367 I used the DAKO Labs' developer kit (with the Biotinylated Link and Streptavidin-HRP) and DAB chromogen for the staining, and Mayer's Hemotoxylin for the counter-stain. The results were not very good. The Pharmingen ...

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by wetheril
Fri Mar 30, 2007 8:48 pm
 
Forum: Molecular Biology
Topic: Immunohistochemistry HELP--paraffin vs frozen sections
Replies: 0
Views: 2565

The Fiber Disease

... then subjected to tyramide signal amplification according to the manufacturer's instructions (TSA indirect, NEN Life Sciences, Boston, MA). Cy5-streptavidin (Amersham, Piscataway, NJ) at 1 mg/mL was diluted 1:500 and added to the slides for fluorescence signal detection. Tissue sections were ...

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by al
Mon Dec 25, 2006 1:39 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5482628
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