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Streak plate technique

streak plate technique

This is a common microbiological technique for the isolation of bacterial cultures . This technique involves streaking a loopful of bacteria across a solid medium, making sure that the cells are spread over the surface so that when allowed to grow and reproduce each individual cell will, then, give rise to a colony of genetically identical cells - a clonal population. Each colony will then serve as the source for a pure colony of genetically identical cells. This technique is essential for isolating clonal populations of bacteria in order to determine the number, and to identify bacteria present in a particular sample.

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How do you grow an isolated colony of e.coli from scratch?

Using A-septic technique, flame sterilize your inoculating loop and transfer your organism to a nutrient agar or bhi plate (not sure but I think ecoli will grow on any media). To do a streak plate, which is used to isolate a mixed culture into ...

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by mikki
Wed Nov 22, 2006 7:28 am
Forum: Microbiology
Topic: How do you grow an isolated colony of e.coli from scratch?
Replies: 4
Views: 21605

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