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Streak culture

streak culture

A culture produced by lightly stroking an inoculating needle or loop with inoculum over the surface of a solid medium.


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URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!

... cells. SPORE REACTION (-) ACID FAST STAINING (-) NUTRIENT BROTH CULTURE- Quite turbid, slightly cloudy, no skin NUTRIENT AGAR STREAK- Shape – Cocci, Yellow, 0.2-0.5m Glossy NUTRIENT AGAR STAB: Aerobic GELATIN STAB: No ...

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by microbiologyhelp
Wed Nov 20, 2013 6:09 pm
 
Forum: Microbiology
Topic: URGENT HELP IDENTIFYING UNKNOWN BACTERIA!!!
Replies: 6
Views: 5398

can old culture of s. aureus not ferment mannitol

Is it possible for an old culture of s. Aureus to not be able to ferment mannitol (on an MSA plate) efficiently? The first time that I streak my unknown on an MSA plate, there was growth and fermentation of mannitol. Through other biochemical ...

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by thuydeebird
Sat Apr 21, 2012 6:22 pm
 
Forum: Microbiology
Topic: can old culture of s. aureus not ferment mannitol
Replies: 0
Views: 755

Re: NEED HELP WITH MY UNKNOWN GRAM POSITIVE ORGANISM PLEASE??

... 4) catalase negative. 5) coagulase negative. I suggest that you re-streak a new mannitol salt agar plate because i think that based on your ... some growth on mannitol. Or it could be that you did not have a pure culture, so in that case you should do a subculture from your "pure ...

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by mariah000
Sun Mar 18, 2012 7:50 pm
 
Forum: Microbiology
Topic: NEED HELP WITH MY UNKNOWN GRAM POSITIVE ORGANISM PLEASE??
Replies: 1
Views: 16934

Microbiology Case Study Help

... inability to differentiate esp among the Gram positive cocci. You would streak for isolation on sheep blood agar. Smaller translucent colonies surrounded ... few tests to confirm but treatment would be initiated based on initial culture observations if not before. The above is the classic approach ...

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by JorgeLobo
Mon Jan 02, 2012 12:50 pm
 
Forum: Microbiology
Topic: Microbiology Case Study Help
Replies: 4
Views: 3887

growing E coli from a starter culture

... can go directly from glycerol to broth. -but if you have enough time streak on a plate and restart from an isolated colony. and ampicillin stinks ... try to go with a first isolation/selection step before restarting a culture.

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by canalon
Tue Apr 22, 2008 1:16 pm
 
Forum: Microbiology
Topic: growing E coli from a starter culture
Replies: 7
Views: 18894
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