Streak culture

Is it possible for an old culture of s. Aureus to not be able to ferment mannitol (on an MSA plate) efficiently? The first time that I streak my unknown on an MSA plate, there was growth and fermentation of mannitol. Through other biochemical ...

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... 4) catalase negative. 5) coagulase negative. I suggest that you re-streak a new mannitol salt agar plate because i think that based on your ... some growth on mannitol. Or it could be that you did not have a pure culture, so in that case you should do a subculture from your "pure ...

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... inability to differentiate esp among the Gram positive cocci. You would streak for isolation on sheep blood agar. Smaller translucent colonies surrounded ... few tests to confirm but treatment would be initiated based on initial culture observations if not before. The above is the classic approach ...

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... can go directly from glycerol to broth. -but if you have enough time streak on a plate and restart from an isolated colony. and ampicillin stinks ... try to go with a first isolation/selection step before restarting a culture.

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I guess the "proper" way is to gouge out a bit of the culture and streak it onto plates (with the appropriate selective media), grow the plate overnight, and then ...

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