Streak culture

... can go directly from glycerol to broth. -but if you have enough time streak on a plate and restart from an isolated colony. and ampicillin stinks ... try to go with a first isolation/selection step before restarting a culture.

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I guess the "proper" way is to gouge out a bit of the culture and streak it onto plates (with the appropriate selective media), grow the plate overnight, and then ...

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... want is to significantly decrease the number of bacteria between each streak (or set of streaks). So the simple reliable and quick method I used ... across the reamining space. I could usually isolate easily 6 different culture on 1 plate with a good reliability.

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... bhi plate (not sure but I think ecoli will grow on any media). To do a streak plate, which is used to isolate a mixed culture into a pure culture (in the form of colonies) use the four-quadrant method. Inoculate ...

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... end up with 8 stocks of identical, dna wise, ecoli. I know i have to streak the ecoli to begin with, grow overnight on plates, and then from the ... recomend preparing 4/6L! of LB in 2 or 3 flasks to add starter culture too! and then grow to arround 0.6 OD, is this correct! that much ...

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